Anti-Collagen XVII 抗体 [EPR14758] (ab186415)
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.co.jp/ps/products/186/ab186415/Images/ab186415-505675-anti-collagen-XVII-antibody-epr14758-immunocytochemistry-a431-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14758] to Collagen XVII
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Collagen XVII antibody [EPR14758]
Collagen XVII 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14758] to Collagen XVII -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human skin. ICC/IF: Human A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14758 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250. |
ターゲット情報
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関連性
Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene. -
細胞内局在
Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane. -
参照データベース
- Entrez Gene: 1308 Human
- Omim: 113811 Human
- SwissProt: Q9UMD9 Human
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別名
- 180 kDa bullous pemphigoid antigen 2 antibody
- Alpha 1 type XVII collagen antibody
- BA16H23.2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Collagen XVII antibody [EPR14758] (ab186415)
ab186415 staining Collagen XVII in A431 (Human epidermoid carcinoma epithelial cell). The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% tritonX-100. The cells were then incubated with ab186415 at 10μg/ml concentration followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used at 1/200 dilution as counterstain (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.co.jp/ps/products/186/ab186415/Images/ab186415-505674-anti-collagen-XVII-antibody-epr14758-immunohistochemistry-skin-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen XVII antibody [EPR14758] (ab186415)Immunohistochemical analysis of Paraffin-embedded sections Human skin tissue labelling Collagen XVII with ab186415 at 1/500 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human skin tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Collagen XVII antibody [EPR14758] (ab186415)](https://www.abcam.co.jp/ps/products/186/ab186415/Images/ab186415-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen XVII antibody [EPR14758] (ab186415)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (11)
ab186415 は 11 報の論文で使用されています。
- Samra T et al. Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo. Int J Mol Sci 24:N/A (2023). PubMed: 37958946
- Xiang Y et al. Reduced expression of Collagen 17A1 in naturally aged, photoaged, and UV-irradiated human skin in vivo: Potential links to epidermal aging. J Cell Commun Signal 16:421-432 (2022). PubMed: 35060094
- Wang Y et al. Collagen XVII deficiency alters epidermal patterning. Lab Invest 102:581-588 (2022). PubMed: 35145203
- Watanabe M et al. Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity. Exp Dermatol 30:62-67 (2021). PubMed: 32970880
- Fujimura Y et al. Hair follicle stem cell progeny heal blisters while pausing skin development. EMBO Rep 22:e50882 (2021). PubMed: 34085753