AB260040

Anti-Collagen X 抗体 [EPR22920-211]

Anti-Collagen X antibody [EPR22920-211]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
詳細を見る

(1 Review)

(24 Publications)

Anti-Collagen X antibody [EPR22920-211] (ab260040) is a rabbit monoclonal antibody detecting Collagen X in IHC-P, IHC-Fr. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

Collagen alpha-1(X) chain, COL10A1

2 Images

Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Trition X-100 permeabilized mouse trachea tissue labeling Collagen X with ab260040 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on hyaline cartilage of mouse trachea (PMID : 22859705). The nuclear counterstain is DAPI (blue).

Secondary antibody only control : ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

Immunohistochemical analysis of paraffin-embedded mouse articular cartilage tissue labeling Collagen X with ab260040 at 1/1000 dilution (0.57μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the hypertrophic zone in the growth plate of mouse bone (PMID : 22859705). The section was incubated with ab260040 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : A ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22920-211

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

IHC-P, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Collagen X antibody [EPR22920-211] (ab260040) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

Other related products
We have a range of other formats of antibody clone [EPR22920-211] also available for your convenience: ab260040, Carrier free - ab264571

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Collagen Type X also referred to as Col10 or COL-X is a short-chain collagen specifically a homotrimer which means it is composed of three identical chains. It has an approximate molecular mass of 59 kDa. This collagen is primarily expressed in the hypertrophic zone of growth plate cartilage where endochondral ossification occurs. This specialized location facilitates its function in the process of bone growth and development.

Biological function summary

COL-X plays a critical role in the regulation of chondrocyte maturation during bone formation. It does not function as part of a larger multiprotein complex but acts independently to promote the mineralization of cartilage transitioning cartilage into bone. This activity is essential for proper skeletal morphogenesis particularly in mammals.

Pathways

COL-X participates in the endochondral ossification pathway one of the major pathways that convert cartilage into bone. It's tightly associated with proteins like matrix metalloproteinase 13 (MMP-13) which degrades cartilage matrix and with Indian hedgehog (Ihh) signaling which regulates the proliferation and differentiation of chondrocytes. These proteins collectively ensure the proper progression of chondrocyte hypertrophy and subsequent ossification.

Disruptions in the expression or function of COL-X are linked to conditions like Schmid metaphyseal chondrodysplasia and Osteoarthritis. Schmid metaphyseal chondrodysplasia arises from mutations in the Col10A1 gene impairing skeletal development. In the context of osteoarthritis alterations in COL-X may affect cartilage integrity and joint function. Both conditions involve interactions with type II collagen (COL-II) highlighting the collaborative role of collagens in maintaining cartilage and bone health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

See full target information COL10A1

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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 54:492-508 PubMed40955375

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Inherently bioactive iron-chelating Poly (N-acryloyl 2-glycine)/chitosan hydrogel scaffolds orchestrating dual hypoxic-immune microenvironment for functional meniscus regeneration.

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Bingbing Xu,Jing Ye,Shitang Song,Xueyu Dou,Chao Li,Xing Wang,Jia-Kuo Yu

Chinese medicine 20:142 PubMed40890747

2025

Sodium tanshinone IIA sulfonate alleviates osteoarthritis through targeting SIRT1.

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Mao Xu,Xulei Sun,Xiao Ma,Zixuan Qin,Xin Gao,Xinxin Jin,Hongzhi Sun

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

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Species

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Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

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Species

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Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413970 PubMed39936497

2025

N6-Methyladenosine-Modified circSMAD4 Prevents Lumbar Instability Induced Cartilage Endplate Ossification.

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Unspecified application

Species

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Hanwen Li,Yingchuang Tang,Sihan Hu,Xingbang Ruan,Junxin Zhang,Yihan Shi,Liang Qiu,Huilin Yang,Kai Zhang,Hao Chen,Kangwu Chen

Experimental biology and medicine (Maywood, N.J.) 249:10066 PubMed39911634

2025

Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice.

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Unspecified application

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Marc Teitelbaum,Maya D Culbertson,Charlene Wetterstrand,J Patrick O'Connor

Communications biology 7:1380 PubMed39443661

2024

Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.

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Unspecified application

Species

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Zibin Zhou,Xiaoyu Mao,Chun Jiang,Wenguo Li,Tiantian Zhou,Meng Liu,Shijin Sun,Mengting Wang,Ningzheng Dong,Qingyu Wu,Haibin Zhou

Scientific reports 14:20136 PubMed39209899

2024

MiR-322-5p is involved in regulating chondrocyte proliferation and differentiation in offspring's growth plate of maternal gestational diabetes.

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Species

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Fan Qian,Xianlong Chen,Simiao Wang,Yeyin Zhong,Min Liu,Guang Wang,Xuesong Yang,Xin Cheng

eLife 12: PubMed38896465

2024

Senescence of endplate osteoclasts induces sensory innervation and spinal pain.

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Unspecified application

Species

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Dayu Pan,Kheiria Gamal Benkato,Xuequan Han,Jinjian Zheng,Vijay Kumar,Mei Wan,Junying Zheng,Xu Cao

Frontiers in cell and developmental biology 12:1360041 PubMed38895158

2024

Sclerostin modulates mineralization degree and stiffness profile in the fibrocartilaginous enthesis for mechanical tissue integrity.

Applications

Unspecified application

Species

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Shinsei Yambe,Yuki Yoshimoto,Kazutaka Ikeda,Koichiro Maki,Aki Takimoto,Akihide Tokuyama,Shinnosuke Higuchi,Xinyi Yu,Kenta Uchibe,Shigenori Miura,Hitomi Watanabe,Tetsushi Sakuma,Takashi Yamamoto,Kotaro Tanimoto,Gen Kondoh,Masataka Kasahara,Toshihide Mizoguchi,Denitsa Docheva,Taiji Adachi,Chisa Shukunami

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen X 抗体 [EPR22920-211]

Anti-Collagen X antibody [EPR22920-211]

Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (AB260040)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (24)

Abcam product promise