Anti-Collagen VI 抗体 (ab6588)
Key features and details
- Rabbit polyclonal to Collagen VI
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Collagen VI antibody
Collagen VI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Collagen VI -
由来種
Rabbit -
特異性
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals
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免疫原
Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
Database link: P12109 -
ポジティブ･コントロール
- WB: HepG2 whole cell lysate.
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特記事項
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6588の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa). This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
ターゲット情報
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機能
Collagen VI acts as a cell-binding protein. -
関連疾患
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
配列類似性
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 1291 Human
- Entrez Gene: 1292 Human
- Entrez Gene: 1293 Human
- Entrez Gene: 12833 Mouse
- Entrez Gene: 12834 Mouse
- Entrez Gene: 12835 Mouse
- Entrez Gene: 294337 Rat
- Entrez Gene: 361821 Rat
see all -
別名
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
see all
画像
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Western blot - Anti-Collagen VI antibody (ab6588)Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 108 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (151)
ab6588 は 151 報の論文で使用されています。
- Kanazawa Y et al. Effects of Aging on Collagen in the Skeletal Muscle of Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37685934
- Riddell DO et al. Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy. Dis Model Mech 16:N/A (2023). PubMed: 38050706
- Moammer H et al. Pirfenidone increases transverse tubule length in the infarcted rat myocardium. Interface Focus 13:20230047 (2023). PubMed: 38106917
- Janbandhu V et al. Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction. Cell Stem Cell 29:281-297.e12 (2022). PubMed: 34762860
- Engel AL et al. Extracellular matrix dysfunction in Sorsby patient-derived retinal pigment epithelium. Exp Eye Res 215:108899 (2022). PubMed: 34929159