Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Collagen VI alpha 2 抗体 [EPR7889(N)] (ab180855)

Reviews (1) Submit a question References (3)
Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
  • Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Collagen VI alpha 2 antibody [EPR7889(N)]
    Collagen VI alpha 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7889(N)] to Collagen VI alpha 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Collagen VI alpha 2 aa 800-900 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P12110

  • ポジティブ･コントロール

    • WB: Human skin lysate IHC-P: Human colon carcinoma tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR7889(N)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180855の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified form, use 1/500 - 1/1000 dilution
WB
1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
特記事項
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified form, use 1/500 - 1/1000 dilution
WB
1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Collagen VI acts as a cell-binding protein.

  • 関連疾患

    Defects in COL6A2 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A2 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
    Defects in COL6A2 are the cause of myosclerosis autosomal recessive (MYOSAR) [MIM:255600]; also known as myosclerotic myopathy or congenital myosclerosis of Lowenthal. A condition characterized by chronic inflammation of skeletal muscle with hyperplasia of the interstitial connective tissue. The clinical picture includes slender muscles with firm 'woody' consistency and restriction of movement of many joints because of muscle contractures.

  • 配列類似性

    Belongs to the type VI collagen family.
    Contains 3 VWFA domains.

  • 翻訳後修飾

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix. Membrane. Recruited on membranes by CSPG4.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CO6A2_HUMAN antibody
      • COL6A2 antibody
      • Collagen alpha 2(VI) chain antibody
      • Collagen alpha-2(VI) chain antibody
      • collagen type VI alpha 2 antibody
      • Collagen VI alpha 2 polypeptide antibody
      • human mRNA for collagen VI alpha 2 C terminal globular domain antibody
      • PP3610 antibody
      see all

    画像

    • Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
      Western blot - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
      Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855) at 1/1000 dilution (Purified) + Human skin lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 109 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Collagen VI alpha 2 with Purified ab180855 at 1:4000 dilution (0.413 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
    • Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
      Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)

    参考文献 (3)

    ab180855 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180855 は 3 報の論文で使用されています。

    • Chen PY  et al. Comprehensive Transcriptomic Analysis and Experimental Validation Identify lncRNA HOXA-AS2/miR-184/COL6A2 as the Critical ceRNA Regulation Involved in Low-Grade Glioma Recurrence. Onco Targets Ther 13:4999-5016 (2020). PubMed: 32581558
    • Endicott J  et al. Authentication of collagen VI antibodies. BMC Res Notes 10:358 (2017). PubMed: 28755659
    • Shaheen NL  et al. Extracellular matrix composition modulates angiosarcoma cell attachment and proliferation. Oncoscience 4:178-188 (2017). PubMed: 29344556

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI alpha 2 antibody [EPR7889(N)]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 21 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com