Anti-Collagen V 抗体 (ab7046)
Key features and details
- Rabbit polyclonal to Collagen V
- Suitable for: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICC
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Collagen V antibody
Collagen V 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Collagen V -
由来種
Rabbit -
特異性
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
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アプリケーション
適用あり: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICCmore details -
種交差性
交差種: Mouse, Cow, Human -
免疫原
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
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特記事項
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
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| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
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| IHC-P | (6) |
1/50 - 1/200.
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| IP |
Use at an assay dependent concentration.
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration. PubMed: 18385800
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| 特記事項 |
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WB
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended. |
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Sandwich ELISA
Use at an assay dependent concentration. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
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IHC-P
1/50 - 1/200. |
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. PubMed: 18385800 |
ターゲット情報
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機能
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. -
関連疾患
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2 -
配列類似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain. -
ドメイン
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 282192 Cow
- Entrez Gene: 1289 Human
- Entrez Gene: 1290 Human
- Entrez Gene: 50509 Human
- Entrez Gene: 12831 Mouse
- Entrez Gene: 12832 Mouse
- Entrez Gene: 53867 Mouse
- Omim: 120190 Human
see all -
別名
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
see all
画像
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Western blot - Anti-Collagen V antibody (ab7046)Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (56)
ab7046 は 56 報の論文で使用されています。
- McCourt JL et al. Multi-omics analysis of sarcospan overexpression in mdx skeletal muscle reveals compensatory remodeling of cytoskeleton-matrix interactions that promote mechanotransduction pathways. Skelet Muscle 13:1 (2023). PubMed: 36609344
- Xu Y et al. Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction. Int J Mol Sci 24:N/A (2023). PubMed: 37176085
- Horiba S et al. M1/M2 Macrophage Skewing is Related to Reduction in Types I, V, and VI Collagens with Aging in Sun-Exposed Human Skin. JID Innov 3:100222 (2023). PubMed: 37789949
- Maamra M et al. Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling. Br J Pharmacol 179:2697-2712 (2022). PubMed: 34879432
- Sterken BA et al. C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells. NPJ Breast Cancer 8:11 (2022). PubMed: 35042889