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Anti-Collagen III 抗体 [1E7-D7/Col3] (ab23445)

Reviews (4)Q&A (2)References (28)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)

    Key features and details

    • Mouse monoclonal [1E7-D7/Col3] to Collagen III
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Collagen III antibody [1E7-D7/Col3]
      Collagen III 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [1E7-D7/Col3] to Collagen III

    • 由来種

      Mouse

    • 特異性

      ab23445 is highly specific for type III Collagen. It has been shown to have no cross reactivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      非交差種: Sheep, Cow

    • 免疫原

      Full length native protein (purified) corresponding to Human Collagen III.
      Database link: P02461

    • エピトープ

      The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.

    • ポジティブ･コントロール

      • Frozen and unfixed human or kangaroo skin tissue slides.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab23445の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (2)
    Use at an assay dependent concentration.
    特記事項
    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Collagen type III occurs in most soft connective tissues along with type I collagen.

    • 関連疾患

      Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
      Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
      Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.

    • 配列類似性

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.

    • 翻訳後修飾

      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Alpha 1 type III collagen antibody
        • Alpha1 (III) collagen antibody
        • CO3A1_HUMAN antibody
        • COL 3A1 antibody
        • COL3A1 antibody
        • Collagen alpha 1(III) chain antibody
        • Collagen alpha-1(III) chain antibody
        • Collagen III alpha 1 chain precursor antibody
        • Collagen III alpha 1 polypeptide antibody
        • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
        • Collagen type III alpha 1 antibody
        • Collagen type III alpha 1 chain antibody
        • Collagen type III alpha antibody
        • Collagen, fetal antibody
        • EDS4A antibody
        • Ehlers Danlos syndrome type IV, autosomal dominant antibody
        • Fetal collagen antibody
        • Type III collagen antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)

        Immunohistochemistry (Paraffin-embedded sections) analysis of human bronchus tissue labelling Collagen III with ab23445.

      参考文献 (28)

      ab23445 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab23445 は 28 報の論文で使用されています。

      • Esper ME  et al. Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease. Methods Mol Biol 2566:113-132 (2023). PubMed: 36152246
      • Lee JH  et al. Adipose Tissue-Derived Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing and Tissue Regeneration. Int J Mol Sci 24:N/A (2023). PubMed: 37445612
      • Bérniz C  et al. Breast Implant Capsule: A Murine Model Comparing Capsular Contracture Susceptibility Among Six Breast Implants Available in the Market. Aesthetic Plast Surg 47:2093-2105 (2023). PubMed: 37022396
      • Su Y  et al. Photocatalytic oxygen evolution and antibacterial biomimetic repair membrane for diabetes wound repair via HIF1-α pathway. Mater Today Bio 20:100616 (2023). PubMed: 37025556
      • Passanha FR  et al. Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGFβ1 Pathway. Stem Cells 40:669-677 (2022). PubMed: 35416252
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [1E7-D7/Col3]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: cirtric acid
      Permeabilization
      No
      Specification
      skin
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 02 2020

      Western blot abreview for Anti-Collagen III antibody [1E7-D7/Col3]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Tissue lysate - whole (skin)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Treatment
      burn wound with stem cell treatment
      Specification
      skin
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 May 20 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [1E7-D7/Col3]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 0.05% typsin
      Sample
      Human Tissue sections (huam whole fat tissue)
      Specification
      huam whole fat tissue
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 13 2015

      Immunohistochemistry (Frozen sections) abreview for Anti-Collagen III antibody [1E7-D7/Col3]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Pig Tissue sections (pig skin frozen sections)
      Specification
      pig skin frozen sections
      Fixative
      Acetone
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 24 2013

      Question

      Thank you for your detailed answer about the anti-collagen III antibody ab6310.

      That's a pity indeed that the validity on pig was initially a mistake but these things happen. I am a regular customer who appreciate very much your policy about customer feedback and reviewing, and I'll submit an abreview about IHC-Fr in pig skin after performing the few more tests discussed.

      In return, to answer your questions:

      - From what I've read and optimized so far on frozen sections with different kind of antibodies, the antigen retrieval step is usually not needed as the fixation time is very short.

      I don't have any pronase, and I'm not sure it's worth for me buying some only for a test, with no insurance the antibody will work. I can try fixing in acetone instead or formalin see if it changes something.

      - I will follow your advice and block with only donkey serum or BSA but not both at the same time

      - The secondary antibody works well, I've written reviews about ab90395 (collagen I) and ab6328 (fibronectin EDA), where I used the same anti-mouse Cy2 secondary antibody on similar pig skin frozen sections with good results.

      - I have performed the procedure on a couple of separate samples, and I did it twice on independent batches before contacting the technical service, to ensure the problem was from the antibody and not from my experimental procedure.

      I’d like to try the reference ab23445, as I also would like to do some western blotting. If this second one doesn't work, will I still be eligible to try the other one, or for a refund?

      Thank you very much

      Best regards

      Read More
      Answer

      Thank you foryour understanding and cooperation. The details provided enable us to closely monitor the quality of our products.

      I hope the suggestions may proove useful with ab6310. It may be worth trying citrate antigen retreival with the PFA fixationif you have the reagents. It is unusual to use antigen retreival with IHC-Fr, however laboratory tests do indicate that this can sometimes improve the results.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedthe alternativefree of charge replacement of ab23445with the order number 1132685.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. The ab23445 is tested and covered by the guarantee for IHC-Fr, WB and pig samples. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Suchen daher monoklonale AK fuer die IHC-P:
      1) gegen Ratte und NICHT gegen Schwein
      2) umgekehrt
      Collagen III oder I
      am liebsten monoklonal (Maus, kann auch Kaninchen sein)

      Read More
      Answer

      Vielen Dank für Ihren Anruf.

      Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, die für Ihre Versuchsvorhaben geeignet sein könnten:

      ab23446:mouse monoclonalanti-Collagen I antibody, reacts with pig, does not react with mouse. Daherbesteht die Möglichkeit, dass er nicht mit Ratte kreuzreagiert. Bisher wurde dies nicht getestet.

      ab88147: Mouse monoclonal Anti-Collagen I antibody, reacts with human andmouse. Sequenzähnlichkeit des Immunogens gegenüber Schwein: 77%, gegenüber Ratte: 96%. Es könnte also sein, dass er mit Ratte kreuzreagiert, aber nicht mit Schwein.

      ab82354: Mouse monoclonalAnti-Collagen III antibody, reacts with rat. Aufgrund einer Sequenzähnlichkeit des humanen Kollagen III von weniger als 57% gegen das Ortholog des Schweins ist die Wahrscheinlichkeit einer Kreuzreaktivität sehr gering.

      ab78032: Mouse monoclonal Anti-Collagen III antibody, reacts with human. Aufgrund einer Sequenzähnlichkeit des humanen Kollagen III von weniger als 57% gegen das Ortholog des Schweins ist die Wahrscheinlichkeit einer Kreuzreaktivität sehr gering. Die Sequenzaehnlichkeit gegen Ratte liegt bei 90%; er könnte also mit Ratte funktionieren.

      Für den von Ihnen genannten ab23445 hatte ich im Labor nachgefragt, das uns bestätigt hat, dass dieser Antikörper leider nicht zwischen dem Kollagen III des Schweins bzw. der Ratte diskriminieren kann. Ich habe das Datenblatt entsprechend aktualisiert.

      Einige der Antikörper haben wir bisher nicht an Paraffinschnitten (ICH-P) getestet, aber wir sehen keinen Grund, warum sie nicht für diese Anwendung geeignet sein sollten.

      Mit jedem deroben genannten Antikörper können Sie an unserem 100%igen Abreview-Rabattprogramm teilnehmen: Wenn Sie den Antikörper selbst testen möchten, bekommen Sie einen beliebigen primären Antikörper kostenlos von uns, wenn Sie uns ein Abreview mit dem Testresultat zusenden.Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Gerne stehe ich auch für weitere Fragen zur Verfügung.

      Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

      Read More

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