Anti-Collagen II 抗体

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

ab34712 (1 : 400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.

Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.

Sonomoto K et al. Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold. PLoS One 11:e0153231 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Collagen II antibody (AB34712)

Collagen II immunocytochemistry-immunofluorescence using Anti-Collagen II antibody ab34712. Publication image and figure legend from Sun, Q., Yin, W., et al., 2022, Front Bioeng Biotechnol, PubMed 36177180.

The chondrogenic bioactivity on ADSCs of curcumin-loaded PEG-GelMA microgel (PGMs). (A) Schematic of ADSCs culture on the curcumin-loaded PEG-GelMA microgel (PGMs) and further tri-lineage differentiation. (B) The level of osteogenesis, chondrogenesis, and adipogenesis of ADSCs was assessed by ARS staining, Alcian blue staining, and Oil Red staining, respectively. Scale bar = 100 μm. (C) Immunostaining of Collagen II protein expression in ADSCs cultured on the PGMs and curcumin (5 uM) -loaded PGMs, respectively. Scale bar = 100 μm. (D) Relative expression of chondrogenic marker genes (COL2A1, SOX9, Aggrecan) after chondrogenic induction for 21 days (Mean ± SD; **p < < 0.01; biological replicates n = 3 per group).

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Collagen II antibody (AB34712)

Collagen II immunocytochemistry-immunofluorescence using Anti-Collagen II antibody ab34712. Publication image and figure legend from Berg, V., Sveinbjörnsson, B., et al. 2011, Arthritis Res Ther, PubMed 21192818 .

Expression of collagen type II and aggrecan in cultured human articular chondrocytes. (a) Cells were labelled with polyclonal rabbit anti-human collagen type II and secondary antibody conjugated with Alexa Fluor 594 (red). (b) Cells were labelled with monoclonal mouse anti-human aggrecan and secondary antibody conjugated with Alexa Fluor 488 (green). The nuclei were visualized by Dapi dye (blue). Isotype controls had no staining (not shown).

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Collagen II antibody (AB34712)

Immunocytochemistry-immunofluorescence using Anti-Collagen II antibody, ab34712. Publication image from Hong, K. H. et al., 2019, Adv Sci (Weinh), 31508277. Legend direct from paper.

MSC chondrogenic induction screening with the various combinations of Ad‐peptides with β‐CD/MSCs. Mice were sacrificed at day 21 postinjection. a) Immunohistochemistry analysis for Col II, the representative protein used for chondrogenesis detection (Scale bar = 20 µm). b) Analysis of fluorescence intensities of Col II (n = 3). c) Col II gene expression levels in mice tissues (n = 3). The * on the bar graph indicates the significance (* : p < 0.05) of Col II expression in T50 H50 compared with other groups.

• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-Collagen II antibody (AB34712)

Collagen II immunohistochemistry using Anti-Collagen II antibody ab34712. Publication image and figure legend from Park, H., Hong, J., et al., 2020, Sci Rep, PubMed 33060735.

TAP2 is localized in the chondrocytes and synoviocytes of arthritic joints of rats. FITC-conjugated TAP2 was delivered to the knee joints in rats (n = 3) on day 7 post-MIA injection. After 48 h, the knee joints were immunostained with (a) anti-collagen type II (chondrocytes) or (b) anti-Thy1 (synoviocytes). Scale bar = 100 μm (left). (c) The intensity of FITC-TAP2 staining was quantified in cartilages and synovium. The data are expressed as the mean ± SEM (one-way ANOVA test, ***p < < 0.001, **p < < 0.01 vs. D9).

• WB

CiteAb

Western blot - Anti-Collagen II antibody (AB34712)

Collagen II western blotting using Anti-Collagen II antibody ab34712. Publication image and figure legend from Tao, L., Yang, K., et al. 2024, Sci Rep, PubMed 39191890.

MIA induced ferroptosis of chondrocytes in cell OA model. (A) Chondrocytes were treated with different concentrations of MIA for 12 h and 24 h. Cell activity was measured by CCK-8 assay kit. (B) IL-1β levels after different concentrations of MIA treatment for 24 h. (C) TNF-α levels after different concentrations of MIA treatment for 24 h. (D) The protein expression of collagen II and MMP13 after different concentrations of MIA treatment for 24 h. (E) Expression levels of the proteins in (D) relative to those in the control group. (F) GSH levels after 5 μm MIA treatment for 24 h. (G) MDA levels after 5 μm MIA treatment for 24 h. (H) The protein expression of ferroptosis-related markers after 5 μm MIA treatment for 24 h. (I) Expression levels of the proteins in (H) relative to those in the control group. (J) FerroOrange staining of chondrocytes after 5 μm MIA treatment for 24 h. Experiments were performed in triplicate. Data are presented as the mean ± SD. *p < < 0.05, **p < < 0.01 vs. control cells analyzed using ANOVA followed by Tukey's post hoc tests.

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