AB6308

Anti-Collagen I 抗体 [COL-1]

Name: Anti-Collagen I 抗体 [COL-1] (ab6308) | Abcam
Brand: Abcam Limited
SKU: AB6308
Price: 56000 JPY
Availability: InStock
Rating: 4 (34 reviews)

Anti-Collagen I antibody [COL-1]

Lab Essentials
詳細を見る

(34 Reviews)

(549 Publications)

Anti-Collagen I antibody [COL-1] (ab6308) is a mouse monoclonal antibody detecting Collagen I in Western Blot, IHC-Fr. Suitable for Human.

- Over 460 publications
- Trusted since 2000

別名を表示する

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

4 Images

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

IHC-Fr

AbReview2615****

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.

This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.

AbReview17126****

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

All lanes:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution

All lanes:

Human brain whole tissue lysate at 10 µg

Secondary

All lanes:

An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution

Predicted band size: 139 kDa

true

Exposure time: 24hr

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

Lane 1:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/mL

Lane 2:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/mL

Lane 3:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/mL

Lane 4:

Western blot - Anti-Collagen I antibody [COL-1] (ab6308)

All lanes:

Recombinant human Collagen 1

Predicted band size: 139 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

COL-1

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-Fr, WB

applications

免疫原

Native Full Length Protein corresponding to Cow COL1A1.

P02453

エピトープ

The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.Positive Control: Hu stomach, skin and adrenal gland tissue lysates.Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "3.5-7 µg/mL", "IHCFr-species-notes": "(amplification required).Use on unfixed tissue or acetone fixed tissue." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Deer": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

製品の詳細

Anti-Collagen I antibody [COL-1] (ab6308) is a House Mouse Monoclonal antibody and is validated for use in IHC-Fr, WB.

Anti-Collagen I antibody [COL-1] (ab6308) has been cited over 467 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-Collagen I antibody [COL-1] (ab6308) has high sensitivity and specificity.

Anti-Collagen I antibody [COL-1] (ab6308) has 33 independent reviews from customers.

Anti-Collagen I antibody [COL-1] (ab6308) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: 95kDa) and is sold in 50 uL selling sizes.

Collagen I (COL1A1)is a major component of the extracellular matrix (ECM) and plays a significant role in cancer progression. In the tumor microenvironment, Collagen I can promote tumor growth, invasion, and metastasis by increasing tissue stiffness and facilitating cancer cell migration.

Production of this antibody has been changed on 23^rd June 2016. The following lots are from ascites and are still in stock as of 23^rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Proprietary technique

精製に関する特記事項

Purified from Tissue culture supernatant.

バッファー組成

pH: 7.4 Preservative: 0.0976% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

style

left-column

文献 (549)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70800 PubMed40845179

2025

Decoy Receptor 2 as a Cell Cycle Arrest Biomarker for Predicting Renal Recovery Following Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangling Yi,Liming Wang,Xiao-Yue Wang,Yu Fang,Jiarui Liu,Kehong Chen,Ya-Ni He,Jia Chen

Stem cells international 2025:5572221 PubMed40822849

2025

KLF5 Regulation of Exosome-Derived miR-152-3p From Bone Marrow Stem Cells Improves Ventricular Arrhythmia After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wu,Xin-Yue Zou,Yi-Wen Jiang,Da-Wei Lin,Feng Jiang,Yao-Sheng Wang

Frontiers in immunology 16:1618012 PubMed40821844

2025

CRIF1 gene therapy ameliorates inflammatory bowel disease by suppressing TH17 cells and fibrosis through mitochondrial function regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Sil Park,Hye Yeon Kang,Ha Yeon Jeong,SeungCheon Yang,JeongWon Choi,Sang Hee Cho,Sol Kim,Bo-In Lee,Mi-La Cho

Frontiers in bioengineering and biotechnology 13:1615650 PubMed40791857

2025

Restoration of tendon repair microenvironment by grapefruit exosome-loaded microneedle system for tendinopathy therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Ruiyang Zhang,Ti Zhang,Yuhao Mu,Talante Juma,Xu Li,Hao Li,Quanyi Guo,Yongping Cao

JCI insight 10: PubMed40627450

2025

CAR-engineered cytolytic Tregs reverse pulmonary fibrosis and remodel the fibrotic niche with limited CRS.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Han Jiang,Meng Zhou,Meng-Di Cheng,Sai Chen,Ying-Qiang Guo

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang

Pharmaceuticals (Basel, Switzerland) 18: PubMed40573271

2025

Topical Application of a Collagen Mimetic Peptide Restores Peripapillary Scleral Stiffness Reduced by Ocular Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren K Wareham,Ghazi O Bou Ghanem,Kristin L Clark,Eric Schlumpf,Brian J Del Buono,David J Calkins

International journal of molecular sciences 26: PubMed40429861

2025

Biochemical and Biomechanical Properties of Scaffold-Free Hyaline Cartilage Generated Under Dynamic Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando P S Guastaldi,David M Kostyra,Nichaluk Leartprapun,Seemantini Nadkarni,Mark A Randolph,Robert W Redmond

Bioengineering (Basel, Switzerland) 12: PubMed40428065

2025

Bilayer Type I Atelocollagen Scaffolds for In Vivo Regeneration of Articular Cartilage Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Hun Woo,Bo Keun Lee,Andrew S Kwak,Jin Hyo Yang,Seo Yeon Kim,Man Soo Kim,Ji Chul Yoo

Cell and tissue research 401:129-143 PubMed40387922

2025

The effect of macromolecular crowders on deposition of extracellular matrix in astrocyte cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Sorour Nemati,Michelle Kilcoyne,Dimitrios Zeugolis,Siobhan S McMahon

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen I 抗体 [COL-1]

Anti-Collagen I antibody [COL-1]

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (AB6308)

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

Western blot - Anti-Collagen I antibody [COL-1] (AB6308)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (549)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise