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Anti-COL8A1 抗体 (ab58776)

Reviews (1)Q&A (3)References (5)

Key features and details

  • Rabbit polyclonal to COL8A1
  • Suitable for: ELISA, WB
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-COL8A1 antibody
    COL8A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to COL8A1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rabbit, Human

  • 免疫原

    Synthetic peptide corresponding to Human COL8A1 (C terminal).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab58776の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
    Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.

  • 組織特異性

    Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.

  • 配列類似性

    Contains 1 C1q domain.

  • 翻訳後修飾

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C3orf7 antibody
    • CO8A1_HUMAN antibody
    • COL8A1 antibody
    • Collagen alpha 1(VIII) chain [Precursor] antibody
    • Endothelial collagen antibody
    • MGC9568 antibody
    • Vastatin antibody
    see all

参考文献 (5)

ab58776 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab58776 は 5 報の論文で使用されています。

  • Guo W  et al. Screening and bioinformatical analysis of differentially expressed genes in nasopharyngeal carcinoma. J Cancer 12:1867-1883 (2021). PubMed: 33753985
  • Ma ZH  et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . PubMed: 23170115
  • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . PubMed: 21372207
  • Todorovic V  et al. Detection of differentially expressed basal cell proteins by mass spectrometry. Mol Cell Proteomics 9:351-61 (2010). WB ; Mouse . PubMed: 19955077
  • Zhao Y  et al. siRNA-targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D-limonence treatment in hepatocarcinoma cells. IUBMB Life 61:74-9 (2009). WB ; Mouse . PubMed: 19109829

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-COL8A1 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231, MCF-7)
Gel Running Conditions
Reduced Denaturing (10-20% tris-glycine)
Loading amount
35 µg
Specification
MDA-MB-231, MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Mar 04 2019

Question

Thank you for getting back to me. If you could let me know if there are any changes to the status of the peptide that would be great.

Read More
Answer

Yes, I will let you know.

Thank you so much for your patience and understanding!

Read More

Question

peptide available for sale?

Read More
Answer

Thank you for contacting us.

I have good news: the peptide is available for sale, but is currently backordered for 3 weeks.
Once I have more information about the catalog number and datasheet I will let you know - hopefully by the end of the week.

Thank you for your patience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Dear Sir or Madame, I am working with your col8a1 antibody (ab58776). Is this antibody tested for crossreaction with the alpha 2 chain of collagen VIII, as I have the feeling that some of my results in Western blotting could rather be the alpha 2 than the alpha 1 chain of collagen VIII. Thank you in advance for your answer. Yours sincerely

Read More
Answer

Thank you for your inquiry.  A cross-reaction of this antibody with the collagen alpha2 (VIII) chain was not specifically tested. But I can confirm that a cross-reaction is very unlikely because the immunogen sequence only shares 66% homology with alpha2. We expect a possible cross-reaction from 85% homology and more. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.  

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