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リコンビナントRabMAb

Anti-COL11A1 抗体 [EPR24327-8] (BSA and Azide free) (ab289725)

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Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)
  • Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
  • Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
  • Immunocytochemistry/ Immunofluorescence - Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)
  • Flow Cytometry (Intracellular) - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
  • Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24327-8] to COL11A1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free)
    COL11A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24327-8] to COL11A1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cyt (Intra)more details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A-204 transfected with scrambled siRNA control, whole cell fresh lysate. A-204 whole cell fresh lysate. ICC/IF: A-204 cells. Flow Cyt: HCT 116 cells.

  • 特記事項

    ab289725 is the carrier-free version of ab270946.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24327-8

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289725の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 280 kDa (predicted molecular weight: 181 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 280 kDa (predicted molecular weight: 181 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.

  • 組織特異性

    Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.

  • 関連疾患

    Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable.
    Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.

  • 配列類似性

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 TSP N-terminal (TSPN) domain.

  • 翻訳後修飾

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • COBA1_HUMAN antibody
      • COL11A1 antibody
      • COLL6 antibody
      • Collagen alpha 1 antibody
      • Collagen alpha-1(XI) chain antibody
      • collagen XI alpha 1 antibody
      • collagen XI, alpha 1 polypeptide antibody
      • collagen, type XI, alpha 1 antibody
      • STL2 antibody
      • STL3 antibody
      • XI chain precursor antibody
      see all

    画像

    • Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)
      Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)
    • Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      All lanes : Anti-COL11A1 antibody [EPR24327-8] (ab270946) at 1/1000 dilution

      Lane 1 : A-204 (Human muscle rhabdomyosarcoma) whole cell fresh lysate
      Lane 2 : HCT116 (human colorectal carcinoma epithelial cell) whole cell fresh lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 181 kDa
      Observed band size: 280 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab270946, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer: 5% NFDM/TBST. 

      This blot was developed using a higher sensitivity ECL substrate.

      Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 32950601).

      The ~150 kDa bands are expected to be non-specific bands.

      Negative control: HCT116 (PMID: 25417197).

    • Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      Western blot - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      All lanes : Anti-COL11A1 antibody [EPR24327-8] (ab270946) at 1/1000 dilution

      Lane 1 : A-204 (Human muscle rhabdomyosarcoma) transfected with scrambled siRNA control, whole cell fresh lysate
      Lane 2 : A-204 transfected with siRNA specifically targeti COL11A1, whole cell fresh lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 181 kDa
      Observed band size: 260 kDa why is the actual band size different from the predicted?



      This data was developed using ab270946, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer: 5% NFDM/TBST.

      Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

      Exposure time: 3 minutes.

    • Immunocytochemistry/ Immunofluorescence - Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)
      Immunocytochemistry/ Immunofluorescence - Anti-COL11A1 antibody [EPR24327-8] (BSA and Azide free) (ab289725)

      This data was developed using ab270946, the same antibody clone in a different buffer formulation. 

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (Human Muscle Rhabdomyosarcoma cell line) whole cells labeling COL11A1 with ab270946 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in A-204 cell line. Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab195889) at 1/200 dilution (Red). 

      Negative control: HCT 116 (PMID: 25417197). The nuclear counterstain is DAPI (blue). 

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. 

    • Flow Cytometry (Intracellular) - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      Flow Cytometry (Intracellular) - Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)

      This data was developed using ab270946, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of HCT 116 (Human colorectal carcinoma epithelial cell), Left)/ A-204 (rat muscle rhabdomyosarcoma, Right) fixed with 4% paraformaldehyde permeabilized with 90% methanol cells labelling COL11A1 with ab270946 at 1/500 (Red), compared with a (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 was used as the secondary antibody. 

      Negative control: HCT116 (PMID: 25417197).

    • Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)
      Anti-COL11A1 antibody [EPR24327-8] - BSA and Azide free (ab289725)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289725 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289725 は論文での使用が確認できていません。

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