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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Coilin 抗体 [EPR7843(N)] - N-terminal (ab177466)

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Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
  • Flow Cytometry (Intracellular) - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
  • Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
  • Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal
  • Suitable for: WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Coilin antibody [EPR7843(N)] - N-terminal
    Coilin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra)more details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P38432

  • ポジティブ･コントロール

    • WB: Jurkat, HeLa, MCF7 and HepG2 cell lysates. Flow Cyt (intra): Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177466の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.

  • 組織特異性

    Found in all the cell types examined.

  • 配列類似性

    Belongs to the coilin family.

  • 細胞内局在

    Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CLN 80 antibody
      • CLN80 antibody
      • COIL antibody
      • COIL_HUMAN antibody
      • Coilin antibody
      • Coilin p80 antibody
      • p80 antibody
      • P80 coilin antibody
      see all

    画像

    • Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : COIL knockout HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 63 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab177466 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.

      ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Flow Cytometry (Intracellular) - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      Flow Cytometry (Intracellular) - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)

      Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green). 

       

       

    • Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 63 kDa

    • Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
      Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)

    参考文献 (0)

    ab177466 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177466 は論文での使用が確認できていません。

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