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リコンビナントRabMAb

Anti-COG4 抗体 [EPR10111] (ab154795)

Reviews (2) Submit a question References (1)
Western blot - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
  • Anti-COG4 antibody [EPR10111] (ab154795)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10111] to COG4
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-COG4 antibody [EPR10111]
    COG4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10111] to COG4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa and K562 cell lysates; Human heart and kidney tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154795の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 関連性

    COG4 is a component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. COG4 is required for normal Golgi function.

  • 細胞内局在

    Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.

  • 参照データベース

    • 別名

      • CDG2J antibody
      • COD1 antibody
      • COG 4 antibody
      • COG complex subunit 4 antibody
      • Complexed with Dor1p antibody
      • Component of oligomeric golgi complex 4 antibody
      • Conserved oligomeric Golgi complex protein 4 antibody
      • Conserved oligomeric Golgi complex subunit 4 antibody
      • DKFZp586E1519 antibody
      see all

    画像

    • Western blot - Anti-COG4 antibody [EPR10111] (ab154795)
      Western blot - Anti-COG4 antibody [EPR10111] (ab154795)
      All lanes : Anti-COG4 antibody [EPR10111] (ab154795) at 1/1000 dilution

      Lane 1 : Hep G2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 89 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling COG4 with ab154795 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG4 with ab154795 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154795 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154795 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue using ab154795 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG4 antibody [EPR10111] (ab154795)

      Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154795 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-COG4 antibody [EPR10111] (ab154795)
      Anti-COG4 antibody [EPR10111] (ab154795)

    参考文献 (1)

    ab154795 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154795 は 1 報の論文で使用されています。

    • Yue X  et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-COG4 antibody [EPR10111]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (CHO)
    Gel Running Conditions
    Reduced Denaturing (10% gel, transferred onto nitrocellulose)
    Loading amount
    40000 cells
    Specification
    CHO
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 01 2023

    Immunocytochemistry/ Immunofluorescence abreview for Anti-COG4 antibody [EPR10111]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    投稿 Feb 18 2013

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