Anti-Cofilin 2 抗体 [EPR24519-7] (ab300128)

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Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24519-7] to Cofilin 2
Suitable for: IP, Flow Cyt (Intra), IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-Cofilin 2 antibody [EPR24519-7]
Cofilin 2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR24519-7] to Cofilin 2
由来種

Rabbit
アプリケーション

適用あり: IP, Flow Cyt (Intra), IHC-P, WBmore details
適用なし: ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Hela, Hela transfected with siRNA specifically targeti Cofilin-2, whole, Human skeletal muscle, HeLa, Raji, Mouse heart, Mouse brain, C2C12, Rat heart, Rat brain, His-tagged human Cofilin 2 recombinant protein lysates. IHC-P: Human cardiac and skeletal muscle, Mouse and rat cardiac muscle tissues. Flow Cyt. Intr.: HeLa and C2C12 cells. IP: Raji and Mouse heart.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR24519-7
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Cofilin 2 antibody [EPR24519-7] (BSA and Azide free) (ab300129)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300128の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IP		1/30.
Flow Cyt (Intra)		1/500.
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).

特記事項
IP 1/30.
Flow Cyt (Intra) 1/500.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).

追加情報

Is unsuitable for ICC/IF.

機能

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
組織特異性

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
関連疾患

Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
配列類似性

Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.
翻訳後修飾

The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
細胞内局在

Nucleus matrix. Cytoplasm > cytoskeleton.
Target information above from: UniProt accession Q9Y281 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1073 Human
- Entrez Gene: 12632 Mouse
- Entrez Gene: 366624 Rat
- Omim: 601443 Human
- SwissProt: Q9Y281 Human
- SwissProt: P45591 Mouse
- Unigene: 180141 Human
- Unigene: 276826 Mouse
別名
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
- Cofilin 2 muscle antibody
- Cofilin antibody
- Cofilin muscle antibody
- Cofilin muscle isoform antibody
- Cofilin-2 antibody
- Cofilin2 antibody
- muscle isoform antibody
- NEM 7 antibody
- NEM7 antibody
see all

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

All lanes : Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1 : Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : Hela transfected with siRNA specifically targeting Cofilin-2, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 147 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 37 seconds
Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

All lanes : Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : C2C12 (mouse myoblasts) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

All lanes : Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 37 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

All lanes : Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

All lanes : Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1 : His-tagged human Cofilin 2 recombinant protein 10 ng
Lane 2 : His-tagged human Cofilin 1 recombinant protein 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

This antibody does not cross-react with human Cofilin 1.
Both recombinant proteins were expressed from E.coli expression systems.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human cardiac muscle. The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded human skeletal muscl tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human skeletal muscle (PMID: 24610938). The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cardiac muscle (PMID: 11809832). The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Cofilin 2 with ab300128 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblasts) cells labelling Cofilin 2 with ab300128 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Cofilin 2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg with ab300128 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300128 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 (Inset): Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg

Lane 2 (+): Raji whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300128 in Raji whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 23.684 seconds
Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Cofilin 2 was immunoprecipitated from 0.35 mg Mouse heart tissue lysate 10µg with ab300128 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300128 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 (Inset): Mouse heart tissue lysate 10µg

Lane 2 (+): Mouse heart tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300128 in Mouse heart tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5.5 seconds
Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab300128 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300128 は論文での使用が確認できていません。

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A