Anti-CoCoA 抗体 (ab167237)
Key features and details
- Mouse polyclonal to CoCoA
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CoCoA antibody
CoCoA 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to CoCoA -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein within Human CoCoA aa 1-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Rat brain tissue lysate; CoCoA transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. |
ターゲット情報
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関連性
Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. -
細胞内局在
Cytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm. -
参照データベース
- Entrez Gene: 57658 Human
- Entrez Gene: 246047 Rat
- SwissProt: Q9P1Z2 Human
- SwissProt: Q66HR5 Rat
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別名
- Calcium binding and coiled coil domain 1 antibody
- CALCOCO1 antibody
- Calphoglin antibody
see all
画像
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Western blot - Anti-CoCoA antibody (ab167237)Anti-CoCoA antibody (ab167237) at 1 µg/ml + Rat brain tissue lysate at 50 µg
Secondary
Goat anti-Mouse IgG HRP
at 1/2500 dilution
Predicted band size: 77 kDa -
Western blot - Anti-CoCoA antibody (ab167237)All lanes : Anti-CoCoA antibody (ab167237) at 1 µg/ml
Lane 1 : CoCoA transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
Lane 1 : Goat anti-Mouse IgG HRP
Lane 2 : Goat anti-Mouse IgG HRP
at 1/2500 dilution
Predicted band size: 77 kDa
データシートおよび資料
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Datasheet download
参考文献 (1)
ab167237 は 1 報の論文で使用されています。
- Nthiga TM et al. CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy. EMBO J 39:e103649 (2020). PubMed: 32525583
