Anti-CNTF 抗体 (ab9785)
Key features and details
- Rabbit polyclonal to CNTF
- Suitable for: IHC-FoFr, Sandwich ELISA, Neutralising, ELISA, WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-CNTF antibody
CNTF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CNTF -
由来種
Rabbit -
アプリケーション
適用あり: IHC-FoFr, Sandwich ELISA, Neutralising, ELISA, WBmore details -
種交差性
交差種: Rat -
免疫原
Recombinant full length protein corresponding to Rat CNTF. Highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor)
Database link: P20294 -
ポジティブ・コントロール
- WB: Recombinant human CNTF protein (ab9786).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr |
Use at an assay dependent concentration.
|
|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. |
|
Neutralising |
Use at an assay dependent concentration.
for one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/ml). |
|
ELISA |
Use at an assay dependent concentration.
Allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF. |
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-FoFr
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. |
Neutralising
Use at an assay dependent concentration. for one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/ml). |
ELISA
Use at an assay dependent concentration. Allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. -
組織特異性
Nervous system. -
配列類似性
Belongs to the CNTF family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 25707 Rat
- SwissProt: P20294 Rat
- Unigene: 6067 Rat
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別名
- Ciliary neurotrophic factor antibody
- CNTF antibody
- CNTF_HUMAN antibody
- HCNTF antibody
データシートおよび資料
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Datasheet download
参考文献 (3)
ab9785 は 3 報の論文で使用されています。
- Yang Y et al. SOX2-Upregulated microRNA-30e Promotes the Progression of Esophageal Cancer via Regulation of the USP4/SMAD4/CK2 Axis. Mol Ther Nucleic Acids 23:200-214 (2021). PubMed: 33376627
- Li J et al. Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis. Evid Based Complement Alternat Med 2020:2892917 (2020). PubMed: 32724322
- Zacks DN et al. Activation of signaling pathways and stress-response genes in an experimental model of retinal detachment. Invest Ophthalmol Vis Sci 47:1691-5 (2006). WB ; Rat . PubMed: 16565410