Anti-CNTF 抗体 (ab46172)
Key features and details
- Rabbit polyclonal to CNTF
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CNTF antibody
CNTF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CNTF -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, ELISA, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant human CNTF.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000.
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| ELISA |
Use at an assay dependent concentration.
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|
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
1/1000. |
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. -
組織特異性
Nervous system. -
配列類似性
Belongs to the CNTF family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1270 Human
- Entrez Gene: 12803 Mouse
- Entrez Gene: 25707 Rat
- Omim: 118945 Human
- SwissProt: P26441 Human
- SwissProt: P51642 Mouse
- SwissProt: P20294 Rat
- Unigene: 524920 Human
see all -
別名
- Ciliary neurotrophic factor antibody
- CNTF antibody
- CNTF_HUMAN antibody
- HCNTF antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CNTF antibody (ab46172)ICC/IF image of ab46172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46172, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab46172 は 11 報の論文で使用されています。
- Li X et al. Astrocytic phagocytosis of myelin debris and reactive characteristics in vivo and in vitro. Biol Cell 115:e202300057 (2023). PubMed: 37851997
- Wei W et al. Neurotrophic Treatment Initiated During Early Postnatal Development Prevents the Alzheimer-Like Behavior and Synaptic Dysfunction. J Alzheimers Dis 82:631-646 (2021). PubMed: 34057082
- Li X et al. MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7. Folia Neuropathol 59:67-80 (2021). PubMed: 33969678
- Cheng XQ et al. Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection. Neural Regen Res 15:503-511 (2020). PubMed: 31571662
- Si ZP et al. CNTF and Nrf2 Are Coordinately Involved in Regulating Self-Renewal and Differentiation of Neural Stem Cell during Embryonic Development. iScience 19:303-315 (2019). PubMed: 31404831
