Anti-CNOT3 抗体 (ab154276)
Key features and details
- Rabbit polyclonal to CNOT3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CNOT3 antibody -
製品の詳細
Rabbit polyclonal to CNOT3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit
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免疫原
Recombinant fragment corresponding to Human CNOT3 aa 522-753 (C terminal).
Database link: O75175 -
ポジティブ･コントロール
- IMR32 whole cell lysate; NT2D1, Jurkat and HeLa cell lines; Human H441 xenograft tissue; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154276の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 82 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
The CCR4-NOT complex functions as general transcription regulation complex. -
組織特異性
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
配列類似性
Belongs to the CNOT2/3/5 family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4849 Human
- Entrez Gene: 232791 Mouse
- Entrez Gene: 308311 Rat
- Omim: 604910 Human
- SwissProt: O75175 Human
- SwissProt: Q8K0V4 Mouse
- Unigene: 343571 Human
- Unigene: 358731 Mouse
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別名
- CCR4 associated factor 3 antibody
- CCR4-associated factor 3 antibody
- CCR4-NOT transcription complex subunit 3 antibody
see all
画像
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Western blot - Anti-CNOT3 antibody (ab154276)Anti-CNOT3 antibody (ab154276) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CNOT3 antibody (ab154276)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel: Costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNOT3 antibody (ab154276)Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154276 は 1 報の論文で使用されています。
- Park J et al. UPF1/SMG7-dependent microRNA-mediated gene regulation. Nat Commun 10:4181 (2019). PubMed: 31519907