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Anti-CNBP 抗体 (ab48027)

Reviews (3)Q&A (1)References (4)
Western blot - Anti-CNBP antibody (ab48027)
  • Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
  • Flow Cytometry - Anti-CNBP antibody (ab48027)
  • Western blot - Anti-CNBP antibody (ab48027)
  • Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
  • Western blot - Anti-CNBP antibody (ab48027)

Key features and details

  • Goat polyclonal to CNBP
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CNBP antibody
    CNBP 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to CNBP

  • 由来種

    Goat

  • 特異性

    No crossreaction expected with CCHC domain containing 13.

  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey

  • 免疫原

    Synthetic peptide:

    GESGHLARECTIE

    , corresponding to C terminal amino acids 162-174 of Human CNBP.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Flow Cyt: MCF7 cells ICC/IF: Neuro-2a and MCF2 cells WB: Neuro2a, MCF7, MOLT-4 and Daudi cell lysate; Human skeletal muscle tissue lysate

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab48027の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB (3)
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

1 hour primary incubation is recommended for this product.
特記事項
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

1 hour primary incubation is recommended for this product.

ターゲット情報

  • 機能

    Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

  • 組織特異性

    Present in all tissues examined.

  • 関連疾患

    Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.

  • 配列類似性

    Contains 7 CCHC-type zinc fingers.

  • 細胞内局在

    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CCHC type zinc finger nucleic acid binding protein antibody
    • Cellular nucleic acid binding protein antibody
    • Cellular nucleic acid-binding protein antibody
    • CNBP antibody
    • CNBP_HUMAN antibody
    • CNBP1 antibody
    • DM2 antibody
    • Erythroid differentiation related antibody
    • PROMM antibody
    • Proximal myotonic myopathy nucleic acid binding protein antibody
    • RNF163 antibody
    • Sterol regulatory element binding protein antibody
    • ZCCHC22 antibody
    • Zinc finger protein 273 antibody
    • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
    • Zinc finger protein 9 antibody
    • ZNF9 antibody
    see all

画像

  • Western blot - Anti-CNBP antibody (ab48027)
    Western blot - Anti-CNBP antibody (ab48027)
    All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : MOLT-4 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Neuro2a cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 19 kDa



    Lysate in RIPA buffer. Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
    Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
    Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
  • Flow Cytometry - Anti-CNBP antibody (ab48027)
    Flow Cytometry - Anti-CNBP antibody (ab48027)
    Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Western blot - Anti-CNBP antibody (ab48027)
    Western blot - Anti-CNBP antibody (ab48027)
    Anti-CNBP antibody (ab48027) at 1 µg/ml + Human Skeletal Muscle lysate at 35 µg

    Predicted band size: 19 kDa



    Lysate in RIPA buffer. Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
    Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab48027)
    Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
  • Western blot - Anti-CNBP antibody (ab48027)
    Western blot - Anti-CNBP antibody (ab48027)Image courtesy of an anonymous Abreview.
    All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 19 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 6 minutes

    See Abreview

参考文献 (4)

ab48027 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab48027 は 4 報の論文で使用されています。

  • Wang Y  et al. Circular RNA circDVL1 inhibits clear cell renal cell carcinoma progression through the miR-412-3p/PCDH7 axis. Int J Biol Sci 18:1491-1507 (2022). PubMed: 35280687
  • Coni S  et al. Translational control of polyamine metabolism by CNBP is required for Drosophila locomotor function. Elife 10:N/A (2021). PubMed: 34517941
  • Yang F  et al. Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation. Mol Cancer 18:158 (2019). PubMed: 31718709
  • Antonucci L  et al. CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc. Cell Cycle 13:434-9 (2014). PubMed: 24275942

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-CNBP antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse cell lines)
Loading amount
20 µg
Specification
Mouse cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 23 2012

Western blot abreview for Anti-CNBP antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat cell lines)
Loading amount
20 µg
Specification
Rat cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 22 2012

Western blot abreview for Anti-CNBP antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T HeLa U251 Jurkat U2OS)
Loading amount
20 µg
Specification
293T HeLa U251 Jurkat U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 01 2010

Question

ab83038 and ab48027: Both of these antibodies are aganist CNBP. Your data sheet o each sayu that each antibody has been tried and works in WB abd ELISA. Other applications have not been tried. However, your spec sheets say both give antigen localization to the endoplasmic reticulum and the cytoplasm, suggesting immunoflorescence has been done with these antibodies. I am interested in using these antibodies for immunoflorescence. Have one, both or neither been used for Immunoflorescence? If so, please let me know. If you have any parameters for IF from in house or a customer on either of these antibodies that would be a great help.

Read More
Answer

Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes from the SwissProt entry for the protein regarding where the protein is known to localize, not where it has been imaged by us, per se: http://www.uniprot.org/uniprot/P62633 Since neither of these antibodies have been tested in ICC/IF, I can offer a discount off a future purchase if you buy ab48027 or ab83038 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab48027 or ab83038 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab48027 or ab83038 so please wait for my reply before ordering. 2. Purchase ab48027 or ab83038 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC/IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab48027 or ab83038 turn out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. If you would like to choose 1 of these antibodies to test, let me know which and I will set you up in our program. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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