Anti-Clusterin beta chain 抗体 [EPR17538-101] (ab184099)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17538-101] to Clusterin beta chain
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Mouse, Rat

Carrier Free

製品名

Anti-Clusterin beta chain antibody [EPR17538-101]
Clusterin beta chain 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR17538-101] to Clusterin beta chain
由来種

Rabbit
アプリケーション

適用あり: IHC-P, WBmore details
種交差性

交差種: Mouse, Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Mouse and rat serum, testis, lung, adrenal gland and stomach lysate. IHC-P: Mouse spleen tissue; rat adrenal gland tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR17538-101
アイソタイプ

IgG
研究分野
- Neuroscience
- Diseases

Alternative Versions
- Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (ab229127)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab184099の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Predicted molecular weight: 52 kDa.

特記事項
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 52 kDa.

機能

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
組織特異性

Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
配列類似性

Belongs to the clusterin family.
翻訳後修飾

Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
細胞内局在

Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
Target information above from: UniProt accession P10909 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
別名
- 40 antibody
- Aging-associated gene 4 protein antibody
- Apo-J antibody
- ApoJalpha antibody
- ApoJbeta antibody
- Apolipoprotein J antibody
- CLI antibody
- CLU antibody
- CLUS_HUMAN antibody
- Clusterin alpha chain antibody
- Complement cytolysis inhibitor a chain antibody
- Complement cytolysis inhibitor antibody
- Complement cytolysis inhibitor b chain antibody
- Complement-associated protein SP-40 antibody
- Ku70-binding protein 1 antibody
- NA1/NA2 antibody
- Testosterone-repressed prostate message 2 antibody
- TRPM-2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded (A) Testis tissue from wild-type C57BL/6J mice (B) Testis tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Testis tissue from wild-type C57BL/6J mice and no staining on (B) Testis tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6J mice (B) Spleen tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6J mice and no staining on (B) Spleen tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded (A) Adrenal gland tissue from wild-type C57BL/6J mice (B) Adrenal gland tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Adrenal gland tissue from wild-type C57BL/6J mice and no staining on (B) Adrenal gland tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).
Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

Lane 1 : Wild-type mouse adrenal gland tissue lysate (male)
Lane 2 : Clusterin knockout adrenal gland tissue lysate (male)
Lane 3 : Wild-type mouse spleen tissue lysate (male)
Lane 4 : Clusterin knockout spleen tissue lysate (male)
Lane 5 : Wild-type mouse testis tissue lysate (male)
Lane 6 : Clusterin knockout testis tissue lysate (male)
Lane 7 : Wild-type mouse adrenal gland tissue lysate (female)
Lane 8 : Clusterin knockout adrenal gland tissue lysate (female)
Lane 9 : Wild-type mouse spleen tissue lysate (female)
Lane 10 : Clusterin knockout spleen tissue lysate (female)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID: T014419).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla than the cortex of rat adrenal gland (PMID: 16436671) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

Lane 1 : Mouse serum
Lane 2 : Rat serum
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible immature (unprocessed))

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2: 5 seconds.

Lane 3: 3 seconds.

Lane 4: 1 minute.

The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).
Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

All lanes : Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099) at 1/1000 dilution

Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse adrenal gland tissue lysate
Lane 3 : Rat adrenal gland tissue lysate
Lane 4 : Rat lung tissue lysate
Lane 5 : Mouse stomach tissue lysate
Lane 6 : Rat stomach tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible immature (unprocessed))

Blocking and dilution buffer: 5% NFDM/TBST .

Exposure times.

Lanes 1, 2 & 3: 3 minutes.

Lane 4: 53 seconds.

Lanes 5 & 6: 100 seconds.

The molecular masses observed are consistent with what has been described in the literature (PMID: 8373947).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of mouse spleen (PMID: 24865838) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Clusterin beta chain antibody [EPR17538-101] (ab184099)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab184099 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184099 は 1 報の論文で使用されています。

Song Z et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (1)

レビューと Q&A