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リコンビナントRabMAb

Anti-CLPX 抗体 [EP8772] (ab168338)

Reviews (2) Submit a question References (9)
Western blot - Anti-CLPX antibody [EP8772] (ab168338)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)
  • Anti-CLPX antibody [EP8772] (ab168338)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP8772] to CLPX
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

製品の概要

  • 製品名

    Anti-CLPX antibody [EP8772]
    CLPX 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP8772] to CLPX

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab168338の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.

  • 組織特異性

    Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

  • 配列類似性

    Belongs to the ClpX chaperone family.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
    • ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
    • AU014732 antibody
    • Caseinolytic mitochondrial matrix peptidase chaperone subunit antibody
    • Caseinolytic peptidase X (E.coli) antibody
    • Caseinolytic protease X antibody
    • clpX antibody
    • ClpX caseinolytic peptidase X homolog antibody
    • ClpX caseinolytic protease X homolog antibody
    • CLPX_HUMAN antibody
    • E330029I21 antibody
    • Energy dependent regulator of proteolysis antibody
    see all

画像

  • Western blot - Anti-CLPX antibody [EP8772] (ab168338)
    Western blot - Anti-CLPX antibody [EP8772] (ab168338)
    All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution

    Lane 1 : A673 lysates
    Lane 2 : HT-1080 lysates
    Lane 3 : Saos-2 lysates
    Lane 4 : Human fetal liver lysates
    Lane 5 : Human fetal heart lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 69 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)
    Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (ab168338)
    Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue).
  • Anti-CLPX antibody [EP8772] (ab168338)
    Anti-CLPX antibody [EP8772] (ab168338)

参考文献 (9)

ab168338 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab168338 は 9 報の論文で使用されています。

  • Torres-Odio S  et al. Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis. J Immunol 206:1890-1900 (2021). PubMed: 33731338
  • Hu D  et al. UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease. Biochem Biophys Res Commun 569:17-22 (2021). PubMed: 34216993
  • Nomura K  et al. Heme-dependent recognition of 5-aminolevulinate synthase by the human mitochondrial molecular chaperone ClpX. FEBS Lett 595:3019-3029 (2021). PubMed: 34704252
  • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
  • Hu D  et al. Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. Acta Neuropathol 137:939-960 (2019). PubMed: 30877431
  • Huff LP  et al. Polymerase-d-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells. Am J Physiol Cell Physiol 316:C621-C631 (2019). PubMed: 30726115
  • Bao Y  et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
  • Franco-Iborra S  et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis 9:1122 (2018). PubMed: 30405116
  • Kubota Y  et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). PubMed: 27496948

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-CLPX antibody [EP8772]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Nothobranchius furzeri Tissue lysate - whole (Liver, Brain, Skeletal muscle, Heart)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, Brain, Skeletal muscle, Heart
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 21 2022

Western blot abreview for Anti-CLPX antibody [EP8772]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse brain, mouse liver, mouse testis)
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Loading amount
20 µg
Specification
mouse brain, mouse liver, mouse testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jana Key

Verified customer

投稿 Apr 22 2022

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