Anti-CLPX 抗体 [EP8772] (ab168338)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP8772] to CLPX
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CLPX antibody [EP8772]
CLPX 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP8772] to CLPX -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A673, HT-1080, Saos-2, Human fetal liver & heart lysates, A673 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP8772 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
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ICC/IF |
1/100 - 1/250.
|
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP. -
組織特異性
Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas. -
配列類似性
Belongs to the ClpX chaperone family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 10845 Human
- Entrez Gene: 270166 Mouse
- Entrez Gene: 300786 Rat
- SwissProt: O76031 Human
- SwissProt: Q9JHS4 Mouse
- SwissProt: Q5U2U0 Rat
- Unigene: 113823 Human
- Unigene: 30088 Mouse
see all -
別名
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
see all
画像
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All lanes : Anti-CLPX antibody [EP8772] (ab168338) at 1/1000 dilution
Lane 1 : A673 lysates
Lane 2 : HT-1080 lysates
Lane 3 : Saos-2 lysates
Lane 4 : Human fetal liver lysates
Lane 5 : Human fetal heart lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa -
Immunofluorescence analysis of A673 cells (red) labeling CLPX with ab168338 at 1/100 dilution. DAPI nuclear staining (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab168338 は 9 報の論文で使用されています。
- Torres-Odio S et al. Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis. J Immunol 206:1890-1900 (2021). PubMed: 33731338
- Hu D et al. UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease. Biochem Biophys Res Commun 569:17-22 (2021). PubMed: 34216993
- Nomura K et al. Heme-dependent recognition of 5-aminolevulinate synthase by the human mitochondrial molecular chaperone ClpX. FEBS Lett 595:3019-3029 (2021). PubMed: 34704252
- Maio N et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
- Hu D et al. Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. Acta Neuropathol 137:939-960 (2019). PubMed: 30877431
- Huff LP et al. Polymerase-d-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells. Am J Physiol Cell Physiol 316:C621-C631 (2019). PubMed: 30726115
- Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
- Franco-Iborra S et al. Defective mitochondrial protein import contributes to complex I-induced mitochondrial dysfunction and neurodegeneration in Parkinson's disease. Cell Death Dis 9:1122 (2018). PubMed: 30405116
- Kubota Y et al. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem N/A:N/A (2016). PubMed: 27496948