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AB188141

Anti-CLK2 抗体

Anti-CLK2 antibody

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(4 Publications)

Rabbit Polyclonal CLK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Dual specificity protein kinase CLK2 aa 1-100.

別名を表示する

Dual specificity protein kinase CLK2, CDC-like kinase 2, CLK2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)

Paraffin embedded human skeletal muscle tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in myocytes cells as expected.

Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (AB188141)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CLK2 antibody (AB188141)

Immunocytochemical analysis of PFA/Triton X-100 fixed U-2 OS cells labeling CLK2 with ab188141 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)

Paraffin embedded human colon tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)

Paraffin embedded human skin tissue stained for CLK2 using ab188141 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLK2 antibody (AB188141)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling CLK2 with ab188141 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CLK2 antibody (AB188141)
  • WB

Supplier Data

Western blot - Anti-CLK2 antibody (AB188141)

All lanes:

Western blot - Anti-CLK2 antibody (ab188141) at 0.4 µg/mL

All lanes:

BeWo (human placenta choriocarcinoma cell line) cell lysate

Predicted band size: 60 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human Dual specificity protein kinase CLK2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P49760

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates : PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed : 28289210).
See full target information Dual specificity protein kinase CLK2
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 69:657-669 PubMed35293765

2022

CDC Like Kinase 2 plays an oncogenic role in colorectal cancer via modulating the Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Bo Zhou,Li Yang,Shi-Fang Liu,Xin-Hua Xu,Zhuo Chen,Yun-Xiao Li,Jin-Hua Yuan,Yong-Chang Wei

American journal of cancer research 10:3765-3783 PubMed33294266

2020

Depletion of CLK2 sensitizes glioma stem-like cells to PI3K/mTOR and FGFR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Soon Young Park,Sandeep Mittal,Jianwen Dong,Kangjin Jeong,Emmanuel Martinez-Ledesma,Yuji Piao,Sabbir Khan,Verlene Henry,Roel Gw Verhaak,Nazanin Majd,Veerakumar Balasubramaniyan,John F de Groot

Proceedings of the National Academy of Sciences of 114:E2644-E2653 PubMed28289210

2017

Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Prakash Kulkarni,Mohit Kumar Jolly,Dongya Jia,Steven M Mooney,Ajay Bhargava,Luciane T Kagohara,Yihong Chen,Pengyu Hao,Yanan He,Robert W Veltri,Alexander Grishaev,Keith Weninger,Herbert Levine,John Orban

Oncotarget 7:26793-805 PubMed27050366

2016

Cdc2-like kinase 2 is a key regulator of the cell cycle via FOXO3a/p27 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Soon Young Park,Yuji Piao,Craig Thomas,Gregory N Fuller,John F de Groot
View all publications
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