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Anti-CLIP170 抗体 [mAbcam61830] (ab61830)

Submit a review Submit a question References (1)
Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)

Key features and details

  • Mouse monoclonal [mAbcam61830] to CLIP170
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CLIP170 antibody [mAbcam61830]
    CLIP170 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [mAbcam61830] to CLIP170

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment corresponding to Human CLIP170 aa 1100 to the C-terminus.
    (Peptide available as ab96632)

  • ポジティブ･コントロール

    • WB: Human skeletal muscle tissue lysate. ICC/IF: HEK293 cells. IHC-P: Human Cervical carcinoma tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab61830の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa).
IHC-P
Use at an assay dependent concentration.
特記事項
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa).
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Seems to be a intermediate filament associated protein that links endocytic vesicles to microtubules.

  • 組織特異性

    Highly expressed in the Reed-Sternberg cells of Hodgkin disease.

  • 配列類似性

    Contains 2 CAP-Gly domains.

  • 細胞内局在

    Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CAP GLY domain containing linker protein 1 antibody
    • CAP-Gly domain-containing linker protein 1 antibody
    • CLIP 170 antibody
    • CLIP antibody
    • CLIP-170 antibody
    • CLIP1 antibody
    • CLIP1_HUMAN antibody
    • CYLN1 antibody
    • cytoplasmic linker 1 antibody
    • Cytoplasmic linker protein 1 antibody
    • Cytoplasmic linker protein 170 alpha 2 antibody
    • Cytoplasmic linker protein 170 alpha-2 antibody
    • Cytoplasmic linker protein CLIP 170 antibody
    • Reed Steinberg cell expressed intermediate filament associated antibody
    • Reed Sternberg intermediate filament associated protein 3 antibody
    • Reed-Sternberg intermediate filament-associated protein antibody
    • Restin antibody
    • RSN antibody
    see all

画像

  • Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Anti-CLIP170 antibody [mAbcam61830] (ab61830) at 1 µg/ml + Human Skeletal Muscle at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Predicted band size: 161 kDa
    Observed band size: 150,161,190 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    Blocking buffer: 3% Milk

    Gel type: TA

  • Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)

    ICC/IF image of ab61830 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61830 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, HepG2, and MCF-7 cell lines in PFA at 10ug/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)
    IHC image of Ab61830 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab61830, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (1)

ab61830 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab61830 は 1 報の論文で使用されています。

  • Li H  et al. Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing. Cells 12:N/A (2022). PubMed: 36611882

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