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リコンビナントRabMAb

Anti-CLEC9A 抗体 [EPR24271-117] (BSA and Azide free) (ab300434)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
  • Flow Cytometry (Intracellular) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
  • Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24271-117] to CLEC9A
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free)
    CLEC9A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24271-117] to CLEC9A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details
    適用なし: ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse spleen tissue, HEK-293T cells transfected with a mouse CLEC9A expression vector containing a his tag. Flow Cyt (intra): Mouse splenocytes.

  • 特記事項

    ab300434 is a carrier free version of ab300433. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300434の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

  • 組織特異性

    In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

  • 配列類似性

    Contains 1 C-type lectin domain.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C type lectin domain family 9, member A antibody
      • C-type lectin domain family 9 member A antibody
      • CLC9A_HUMAN antibody
      • CLEC9A antibody
      • DNGR1 antibody
      • HEEE9341 antibody
      • PRO34046 antibody
      • UNQ9341 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

      This data was developed using ab300433, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling CLEC9A with ab300433 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse spleen is observed. The section was incubated with ab300433 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

      This data was developed using ab300433, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a mouse CLEC9A expression vector containing a his tag and (B) HEK-293T cells transfected with empty vector containing a his tag both labelling CLEC9A with ab300433 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on (A) HEK-293T cells transfected with a mouse CLEC9A expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab300433 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

      This data was developed using ab300433, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse testis labelling CLEC9A with ab300433 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: almost no staining on mouse testis is observed. The section was incubated with ab300433 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Flow Cytometry (Intracellular) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
      Flow Cytometry (Intracellular) - Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

      This data was developed using ab300433, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 2% paraformaldehyde fixed and 0.1% Tween-20 permeabilized mouse splenocytes labelling CLEC9A with ab300433 at 1/50 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. 

      Cells were stained with anti-CD11c conjugated to PE-Cy5.5 and anti-CD8a conjugated to Alexa Fluor® 647.
      Gated on CD11c+ cells.

    • Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)
      Anti-CLEC9A antibody [EPR24271-117] (BSA and Azide free) (AB300434)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300434 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300434 は論文での使用が確認できていません。

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