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AB245121

Anti-CLEC9A 抗体 [EPR22324] - BSA and Azide free

Anti-CLEC9A antibody [EPR22324] - BSA and Azide free

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Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CLEC9A in Flow Cytometry, IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CD370, UNQ9341/PRO34046, CLEC9A, C-type lectin domain family 9 member A

5 Images
Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labeling CLEC9A with ab223188 at 1/50 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab223188 (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD3, PE conjugated anti-HLA-DR and BV421 conjugated anti-CD141. Data shown was firstly gated out lymphocytes, then gated on viable CD3(-) HLA-DR (+) APC cells.

CLEC9A is selectively expressed on CD141+DC cells (Q2, ~0.05% in PBMC) and expression pattern was described in literature. (PMID : 20479116).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on dendritic cells (arrows) of human gastric cancer (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human thyoma tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dendritic cells of human thymoma (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged CLEC9A overexpression construct, labeling CLEC9A with ab223188 at 1/500 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (AB245121)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC9A with ab223188 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101). Positive staining on dendritic cells of human tonsil (PMID : 18408006; PMID : 20479116) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB Ready to use (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223188).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22324

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB104909

Anti-CLEC9A antibody [7H11]

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Primary Antibodies

AB104903

Anti-CLEC9A antibody [1F6]

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Primary Antibodies

AB222794

Anti-CLEC9A antibody

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Primary Antibodies

AB104910

Anti-CLEC9A antibody [8F9]

Immunocytochemistry/ Immunofluorescence - Anti-CLEC9A antibody [8F9] (AB104910)

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組合せ製品

Assay Kits

AB309389

Human CLEC9A ELISA Kit

Sandwich ELISA - Human CLEC9A ELISA Kit (AB309389)

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Sample Prep & Detection Kits

AB102850

Alkaline phosphatase Conjugation Kit - Lightning-Link®

Conjugation - Alkaline phosphatase Conjugation Kit - Lightning-Link® (AB102850)

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Sample Prep & Detection Kits

AB154876

Gold Conjugation Kit (80nm, 20 OD)

Conjugation - Gold Conjugation Kit (80nm, 20 OD) (AB154876)

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Primary Antibodies

AB270797

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BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (AB270797)

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Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

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Primary Antibodies

AB271053

Anti-CD208 antibody [EPR24265-8]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (AB271053)

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Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-CLEC9A antibody [EPR22324] - BSA and Azide free (ab245121) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Other related products
We have a range of other formats of antibody clone [EPR22324] also available for your convenience: ab223188, Carrier free - ab245121, Alexa Fluor® 555 - ab313594, Alexa Fluor® 647 - ab315359

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
See full target information CLEC9A
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6009 PubMed40593805

2025

Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.

Applications

Unspecified application

Species

Unspecified reactive species

David Smith,Anna Eichinger,Éanna Fennell,Zijun Y Xu-Monette,Andrew Rech,Julia Wang,Eduardo Esteva,Arta Seyedian,Xiaoxu Yang,Mei Zhang,Dan Martinez,Kai Tan,Minjie Luo,Katherine J Young,Paul G Murray,Christopher Park,Boris Reizis,Vinodh Pillai

Cell 187:2817-2837.e31 PubMed38701783

2024

FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mana Momenilandi,Romain Lévy,Steicy Sobrino,Jingwei Li,Chantal Lagresle-Peyrou,Hossein Esmaeilzadeh,Antoine Fayand,Corentin Le Floc'h,Antoine Guérin,Erika Della Mina,Debra Shearer,Ottavia M Delmonte,Ahmad Yatim,Kevin Mulder,Mathieu Mancini,Darawan Rinchai,Adeline Denis,Anna-Lena Neehus,Karla Balogh,Sarah Brendle,Hassan Rokni-Zadeh,Majid Changi-Ashtiani,Yoann Seeleuthner,Caroline Deswarte,Boris Bessot,Cassandre Cremades,Marie Materna,Axel Cederholm,Masato Ogishi,Quentin Philippot,Omer Beganovic,Mania Ackermann,Margareta Wuyts,Taushif Khan,Sébastien Fouéré,Florian Herms,Johan Chanal,Boaz Palterer,Julie Bruneau,Thierry J Molina,Stéphanie Leclerc-Mercier,Jean-Luc Prétet,Leila Youssefian,Hassan Vahidnezhad,Nima Parvaneh,Kristl G Claeys,Rik Schrijvers,Marine Luka,Philippe Pérot,Jacques Fourgeaud,Céline Nourrisson,Philippe Poirier,Emmanuelle Jouanguy,Stéphanie Boisson-Dupuis,Jacinta Bustamante,Luigi D Notarangelo,Neil Christensen,Nils Landegren,Laurent Abel,Nico Marr,Emmanuelle Six,David Langlais,Tim Waterboer,Florent Ginhoux,Cindy S Ma,Stuart G Tangye,Isabelle Meyts,Nico Lachmann,Jiafen Hu,Mohammad Shahrooei,Xavier Bossuyt,Jean-Laurent Casanova,Vivien Béziat
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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