Anti-Cleaved PARP1 抗体 (ab4830)
Key features and details
- Rabbit polyclonal to Cleaved PARP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cleaved PARP1 antibody
Cleaved PARP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cleaved PARP1 -
由来種
Rabbit -
特異性
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP1 and will recognize Asp 214 and Gly 215. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Cleaved PARP1.
(Peptide available asab10779) -
ポジティブ･コントロール
- WB: THP1 Nuclear Enriched, HeLa Nuclear Enriched, KARPAS-299 and Daudi cell lysate. HeLa and Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP1. The final product is generated by affinity chromatography using a peptide corresponding to the PARP1 cleavage site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4830の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
ターゲット情報
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機能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾
Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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別名
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
see all
画像
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Western blot - Anti-Cleaved PARP1 antibody (ab4830)All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cells treated with Etoposide (1 µM for 16 hours)
Lanes 3 & 5 : HeLa cell lysate
Lane 4 : HeLa cells treated with Etoposide (1 µM for 16 hours)
Lane 6 : HeLa cells treated with Staurosporine (3 µM for 16 hours)
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/14000 dilution
Predicted band size: 85 kDa -
Western blot - Anti-Cleaved PARP1 antibody (ab4830)All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/2000 dilution
Lane 1 : THP1 Nuclear Enriched
Lane 2 : HeLa Nuclear Enriched
Lane 3 : KARPAS-299
Lane 4 : Daudi cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa -
Western blot - Anti-Cleaved PARP1 antibody (ab4830)This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
Lane 1 : Non-induced Jurkat cells
Lane 2 : Induced Jurkat cells
Secondary
All lanes : Goat Anti-Rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 5 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (56)
ab4830 は 56 報の論文で使用されています。
- Xiong Y et al. Small molecule Z363 co-regulates TAF10 and MYC via the E3 ligase TRIP12 to suppress tumour growth. Clin Transl Med 13:e1153 (2023). PubMed: 36639831
- Zhou Z & Zhang H CHAC1 exacerbates LPS-induced ferroptosis and apoptosis in HK-2 cells by promoting oxidative stress. Allergol Immunopathol (Madr) 51:99-110 (2023). PubMed: 36916093
- Constantin TA et al. The CDK7 inhibitor CT7001 (Samuraciclib) targets proliferation pathways to inhibit advanced prostate cancer. Br J Cancer 128:2326-2337 (2023). PubMed: 37076563
- Liu J et al. Proteomic analysis reveals the molecular mechanism of Astragaloside in the treatment of non-small cell lung cancer by inducing apoptosis. BMC Complement Med Ther 23:461 (2023). PubMed: 38102661
- Ling Z et al. LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the ß-catenin pathway. Open Med (Wars) 17:453-462 (2022). PubMed: 35350839