Anti-Cleaved Caspase-3 抗体 (ab2302)
Key features and details
- Rabbit polyclonal to Cleaved Caspase-3
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cleaved Caspase-3 antibody
Cleaved Caspase-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cleaved Caspase-3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
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ポジティブ･コントロール
- WB: HeLa (treated with staurosporine) whole cell lysate. Camptothecin treated Jurkat whole cell lysate. Cleaved Caspase 3 recombinant protein.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody.
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2302の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (15) |
1/50 - 1/500. Detects a band of approximately 17 kDa.
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| 特記事項 |
|---|
|
WB
1/50 - 1/500. Detects a band of approximately 17 kDa. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
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別名
- active caspase 3 antibody
- Apopain antibody
- CASP 3 antibody
see all
画像
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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)This image is courtesy of an anonymous AbreviewAnti-Cleaved Caspase-3 antibody (ab2302) at 1/2000 dilution + Poly I:C treated Melanoma cells at 20 µg
Secondary
Goat anti-rabbit IgG (HRP) at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)All lanes : Anti-Cleaved Caspase-3 antibody (ab2302)
Lane 1 : Untreated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lane 2 : Camptothecin treated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lysates/proteins at 60 µg per lane. -
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (2 uM Staurosporine, 4Hr) at 20 µg
Lane 2 : HeLa whole cell lysate (untreated) at 20 µg
Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg
Secondary
All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
(2 µM) treated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1368)
ab2302 は 1368 報の論文で使用されています。
- Liu Y et al. Dexamethasone improves thymoma-associated myasthenia gravis via the AKT-mTOR pathway. Naunyn Schmiedebergs Arch Pharmacol 397:817-828 (2024). PubMed: 37498332
- Sen A et al. The effects of dexmedetomidine on trauma-induced secondary injury in rat brain. Neurol Res 46:23-32 (2024). PubMed: 37842946
- Jiang L et al. REG3A promotes proliferation and DDP resistance of ovarian cancer cells by activating the PI3K/Akt signaling pathway. Environ Toxicol 39:85-96 (2024). PubMed: 37665173
- Tang H et al. Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancer. Mol Med 29:127 (2023). PubMed: 37710176
- Guo F et al. Circular RNA METTL15/miR-374a-5p/ESCO2 axis induces colorectal cancer development. Acta Biochim Pol 70:541-549 (2023). PubMed: 37715994