JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB167158

Anti-CLCNKB 抗体 [EPR8414(2)]

Anti-CLCNKB antibody [EPR8414(2)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CLCNKB antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Chloride channel protein ClC-Kb, Chloride channel Kb, ClC-K2, CLCNKB

1 Images
Western blot - Anti-CLCNKB antibody [EPR8414(2)] (AB167158)
  • WB

Unknown

Western blot - Anti-CLCNKB antibody [EPR8414(2)] (AB167158)

All lanes:

Western blot - Anti-CLCNKB antibody [EPR8414(2)] (ab167158) at 1/1000 dilution

Lane 1:

Human fetal lung cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Lane 4:

Human fetal kidney cell lysate at 10 µg

Predicted band size: 75 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-CLCNKB antibody [EPR8414(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8414(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide (PubMed : 11734858, PubMed : 12111250). Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed : 11734858, PubMed : 12111250, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679). Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKB : BSND channels display mostly a linear current-voltage relationship controlled by common gate (PubMed : 11734858, PubMed : 12111250, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679). Mediates chloride conductance along nephron segments, namely the thick ascending limb of Henle's loop, convoluted tubule and the collecting duct, contributing to the maintenance of systemic acid-base and electrolyte homeostasis (By similarity). Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (PubMed : 15044642, PubMed : 18310267, PubMed : 19646679).
See full target information Chloride channel protein ClC-Kb
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:15884 PubMed28635961

2017

Hypoxia-induced mobilization of NHE6 to the plasma membrane triggers endosome hyperacidification and chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Fabrice Lucien,Pierre-Paul Pelletier,Roxane R Lavoie,Jean-Michel Lacroix,Sébastien Roy,Jean-Luc Parent,Dominique Arsenault,Kelly Harper,Claire M Dubois
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB236733

Anti-CLCNKB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCNKB antibody (AB236733)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB169766

Anti-CPOX antibody [EPR11927(B)]

RabMAb|Recombinant

Immunoprecipitation - Anti-CPOX antibody [EPR11927(B)] (AB169766)

  • 0
  • 0
Primary Antibodies

AB75768

Anti-SMC1A (phospho S957) antibody [EPR2857Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (AB75768)

  • 0
  • 0
Primary Antibodies

AB154173

Anti-Thimet Oligopeptidase antibody [EPR7651]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] (AB154173)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com