Name: Anti-CLCA1 antibody [EPR12254-88]
Brand: RabMAb
SKU: ab180851
Rating: 5 (3 reviews)

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Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12254-88] to CLCA1
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 647 Carrier Free HRP

製品名

Anti-CLCA1 antibody [EPR12254-88]
CLCA1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR12254-88] to CLCA1
由来種

Rabbit
特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
アプリケーション

適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
Database link: A8K7I4
ポジティブ･コントロール
- Human colon cancer and human small intestine lysates; human colon tissue; A431 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR12254-88
アイソタイプ

IgG
研究分野

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CLCA1 protein (ab114722)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab180851の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		Use at an assay dependent concentration.
IHC-P	(1)	1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250.
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
ICC/IF	(1)	1/50 - 1/100.

特記事項
Flow Cyt (Intra) Use at an assay dependent concentration.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
ICC/IF 1/50 - 1/100.

機能

May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
組織特異性

Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
配列類似性

Belongs to the CLCR family.
Contains 1 VWFA domain.
翻訳後修飾

Glycosylated.
The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
細胞内局在

Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
Target information above from: UniProt accession A8K7I4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1179 Human
- Entrez Gene: 23844 Mouse
- Entrez Gene: 308015 Rat
- Omim: 603906 Human
- SwissProt: A8K7I4 Human
- SwissProt: Q9D7Z6 Mouse
- Unigene: 194659 Human
- Unigene: 33483 Mouse
別名
- CaCC antibody
- CaCC-1 antibody
- CACC1 antibody
- Calcium dependent chloride channel 1 antibody
- Calcium-activated chloride channel family member 1 antibody
- Calcium-activated chloride channel protein 1 antibody
- Calcium-activated chloride channel regulator 1 antibody
- Chloride channel calcium activated family member 1 antibody
- Chloride channel regulator 1 antibody
- CLCA family member 1 chloride channel regulator antibody
- CLCA1 antibody
- CLCA1_HUMAN antibody
- CLCRG1 antibody
- GOB5 antibody
- hCaCC-1 antibody
- hCLCA1 antibody
see all

Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution (purified) + Human small intestine lysates at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 100 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution (purified)

Lane 1 : Rat small intestine lysates
Lane 2 : Mouse small intestine lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 100 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)

All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution (unpurified)

Lane 1 : Human colon cancer lysates
Lane 2 : Human small intestine lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 100 kDa
Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab180851 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180851 は 15 報の論文で使用されています。

Zhao L et al. Mesenchymal-epithelial interaction regulates gastrointestinal tract development in mouse embryos. Cell Rep 40:111053 (2022). PubMed: 35830795
Mahood TH et al. Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice. ACS Omega 6:1171-1189 (2021). PubMed: 33490776
Centeio R et al. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci 22:N/A (2021). PubMed: 34066250
Lee HJ et al. Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging-related kidney injury. Aging Cell 20:e13407 (2021). PubMed: 34118180
Al-Shaibi AA et al. Human AGR2 Deficiency Causes Mucus Barrier Dysfunction and Infantile Inflammatory Bowel Disease. Cell Mol Gastroenterol Hepatol 12:1809-1830 (2021). PubMed: 34237462

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (Intestine)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Buffer

Permeabilization

Specification

Intestine

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 09 2018

Application

Western blot

Sample

Human Tissue lysate - other (Colon, Small Intestine)

Gel Running Conditions

Reduced Denaturing (12)

Loading amount

20 µg

Specification

Colon, Small Intestine

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 16 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Colonoids)

Permeabilization

Yes - 0.3% triton x-100

Specification

Colonoids

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 12 2016

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (15)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CLCA1 antibody [EPR12254-88]

Western blot abreview for Anti-CLCA1 antibody [EPR12254-88]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CLCA1 antibody [EPR12254-88]