Anti-ClC-4 抗体 (ab75008)
Key features and details
- Rabbit polyclonal to ClC-4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ClC-4 antibody -
製品の詳細
Rabbit polyclonal to ClC-4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human ClC-4 (internal sequence).
-
ポジティブ･コントロール
- WB: Extracts from MCF-7 cells ICC/IF: HUVEC cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75008の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 85 kDa.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 85 kDa. |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
-
機能
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. -
組織特異性
Abundant in skeletal muscle and also detectable in brain and heart. -
配列類似性
Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Contains 2 CBS domains. -
細胞内局在
Early endosome membrane. Late endosome membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1183 Human
- Entrez Gene: 12727 Mouse
- Entrez Gene: 60586 Rat
- Omim: 302910 Human
- SwissProt: P51793 Human
- SwissProt: Q61418 Mouse
- SwissProt: P51794 Rat
- Unigene: 495674 Human
see all -
別名
- Chloride channel 4 antibody
- Chloride channel protein 4 antibody
- Chloride channel, voltage sensitive 4 antibody
see all
画像
-
Western blot - Anti-ClC-4 antibody (ab75008)All lanes : Anti-ClC-4 antibody (ab75008) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 100 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (ab75008)Immunofluorescence analysis of ClC-4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab75008 は 1 報の論文で使用されています。
- Wang C et al. Comprehensive analysis and experimental validation reveal elevated CLCN4 is a promising biomarker in endometrial cancer. Aging (Albany NY) 15:8744-8769 (2023). PubMed: 37671947