AB75008

Anti-ClC-4 抗体

Name: Anti-ClC-4 抗体 (ab75008) | Abcam
Brand: Abcam Limited
SKU: AB75008
Price: 76000 JPY
Availability: InStock

Anti-ClC-4 antibody

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(2 Publications)

Rabbit Polyclonal ClC-4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CLCN4.

別名を表示する

H(+)/Cl(-) exchange transporter 4, Chloride channel protein 4, Chloride transporter ClC-4, ClC-4, CLCN4

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

Immunofluorescence analysis of ClC-4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.

Unknown

Western blot - Anti-ClC-4 antibody (AB75008)

All lanes:

Western blot - Anti-ClC-4 antibody (ab75008) at 1/500 dilution

Lane 1:

extracts from MCF-7 cells at 30 µg

Lane 2:

extracts from MCF-7 cells at 30 µg with immunising peptide

Predicted band size: 84 kDa

Observed band size: 100 kDa,45 kDa,85 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human CLCN4. The exact immunogen used to generate this antibody is proprietary information.

P51793

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ClC-4 also known as chloride channel 4 represents a member of the chloride channel family. It functions as an anion transporter facilitating the movement of chloride ions across cellular membranes. ClC-4 is expressed in various tissues including the kidney brain and skeletal muscle. The protein has a molecular weight of around 91 kDa and is encoded by the CLCN4 gene. Through its transmembrane domain operations ClC-4 helps maintain ion homeostasis and cell volume regulation.

Biological function summary

The functions of ClC-4 involve ion exchange activities important for cellular operations. It does not act alone but is part of a larger complex in particular cell types. In neuronal tissues ClC-4 contributes to the modulation of synaptic transmission and neuronal excitability. Its activity affects the electrochemical gradient across cell membranes essential for normal cellular signaling and metabolism.

Pathways

ClC-4 plays a role in regulating systemic chloride homeostasis and acid-base balance. It functions within pathways related to cell signaling and volume regulation. ClC-4 connects to pathways involving other chloride channels such as ClC-3 and ClC-5 which share complementary roles in ion transport and cellular function. This integration helps maintain the overall balance of ionic and pH levels within the body.

Mutations or dysfunctions in ClC-4 link to neurological conditions like X-linked mental retardation and epilepsy. Such diseases suggest that disruptions in chloride ion transport involve broader neural networking issues. ClC-4's involvement in these disorders intersects with proteins such as ClC-3 which also engages in maintaining neuronal chloride homeostasis. Understanding ClC-4 can provide insights into the pathophysiology of these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed : 18063579, PubMed : 23647072, PubMed : 25644381, PubMed : 27550844, PubMed : 28972156). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed : 29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed : 29845874).

See full target information CLCN4

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38920633

2024

The Immune Checkpoint Protein PD-L1 Regulates Ciliogenesis and Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ewud Agborbesong,Xiaogang Li

Aging 15:8744-8769 PubMed37671947

2023

Comprehensive analysis and experimental validation reveal elevated CLCN4 is a promising biomarker in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Wang,Jing Li,Weina Liu,Shiya Li,Yi Zhang,Yanbin Jin,Jinquan Cui

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ClC-4 抗体

Anti-ClC-4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (AB75008)

Western blot - Anti-ClC-4 antibody (AB75008)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise