Anti-ClC-2 抗体 [EPR6492(2)] (ab154798)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)](https://www.abcam.co.jp/ps/products/154/ab154798/Images/ab154798-161311-anti-clc-2-antibody-epr64922-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6492(2)] to ClC-2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ClC-2 antibody [EPR6492(2)]
ClC-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6492(2)] to ClC-2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human ClC-2. The exact sequence is proprietary.
(Peptide available asab218163) -
ポジティブ･コントロール
- HT29 and HeLa whole cell lysate (ab150035); mouse brain, mouse heart, mouse kidney, mouse spleen, rat kidney and rat spleen lysates; Human thyroid gland carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6492(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154798の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
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| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.
ターゲット情報
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機能
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. -
組織特異性
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. -
関連疾患
Defects in CLCN2 are associated with susceptibility to idiopathic generalized epilepsy type 11 (IGE11) [MIM:607628]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Defects in CLCN2 are the cause of childhood absence epilepsy type 3 (ECA3) [MIM:607682]. ECA3 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3 Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop.
Defects in CLCN2 are associated with juvenile absence epilepsy type 2 (JAE2) [MIM:607628]. JAE is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset occurring around puberty, absence seizures, generalized tonic-clonic seizures (GTCS), GTCS on awakening and myoclonic seizures.
Defects in CLCN2 are associated with juvenile myoclonic epilepsy type 8 (EJM8) [MIM:607628]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
配列類似性
Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily.
Contains 2 CBS domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1181 Human
- Entrez Gene: 12724 Mouse
- Entrez Gene: 29232 Rat
- Omim: 600570 Human
- SwissProt: P51788 Human
- SwissProt: Q6IPA9 Human
- SwissProt: Q9R0A1 Mouse
- SwissProt: P35525 Rat
see all -
別名
- Chloride Channel 2 antibody
- Chloride channel protein 2 antibody
- Chloride channel, voltage sensitive 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ClC-2 with ab154798 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)](https://www.abcam.co.jp/ps/products/154/ab154798/Images/ab154798-161313-anti-clc-2-antibody-epr64922-western-blot.jpg)
Western blot - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)All lanes : Anti-ClC-2 antibody [EPR6492(2)] (ab154798) at 1/1000 dilution
Lane 1 : HT29 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Western blot - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)](https://www.abcam.co.jp/ps/products/154/ab154798/Images/ab154798-161312-anti-clc-2-antibody-epr64922-western-blot.jpg)
Western blot - Anti-ClC-2 antibody [EPR6492(2)] (ab154798)All lanes : Anti-ClC-2 antibody [EPR6492(2)] (ab154798) at 1/1000 dilution
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
Lane 3 : mouse kidney lysate
Lane 4 : mouse spleen lysate
Lane 5 : rat kidney lysate
Lane 6 : rat spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Anti-ClC-2 antibody [EPR6492(2)] (ab154798)](https://www.abcam.co.jp/ps/products/154/ab154798/Images/ab154798-4-benefits-of-recombinant-antibodies.png)
Anti-ClC-2 antibody [EPR6492(2)] (ab154798)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154798 は 2 報の論文で使用されています。
- Ding W et al. ClC-2 inhibition prevents macrophage foam cell formation by suppressing Nlrp3 inflammasome activation. Biosci Biotechnol Biochem 84:2096-2103 (2020). PubMed: 32657644
- Sun L et al. The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K/Akt Signaling Pathway. Int J Mol Sci 17:N/A (2016). WB ; Human . PubMed: 27294913