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Anti-Claudin 9 抗体 [YD4E9] - BSA and Azide free (ab187116)

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Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (ab187116)

    Key features and details

    • Rat monoclonal [YD4E9] to Claudin 9 - BSA and Azide free
    • Suitable for: Competitive ELISA, Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2b

    製品の概要

    • 製品名

      Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free
      Claudin 9 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [YD4E9] to Claudin 9 - BSA and Azide free

    • 由来種

      Rat

    • アプリケーション

      適用あり: Competitive ELISA, Flow Cytmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Recombinant full length protein corresponding to Human Claudin 9 aa 1 to the C-terminus.
      Database link: O95484

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • BOSC23 expressing Claudin 9 cDNA and native Claudin 9 expressed in mammalian cells.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.20
      Constituent: 100% PBS

    • キャリア・フリー

      はい
    • Concentration information loading...

    • 精製度

      Protein G purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      YD4E9

    • アイソタイプ

      IgG2b

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab187116の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Competitive ELISA
    Use at an assay dependent concentration.
    Flow Cyt (1)
    Use 1.2µg for 106 cells.

    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
    特記事項
    Competitive ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use 1.2µg for 106 cells.

    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 関連性

      Claudin 9 is a member of the claudin family. It is a four-transmembrane protein with WWCC motif and plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

    • 細胞内局在

      Cell Membrane (tight junctions)

    • 参照データベース

      • 別名

        • claudin 9 antibody

      画像

      • Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (ab187116)
        Flow Cytometry - Anti-Claudin 9 antibody [YD4E9] - BSA and Azide free (ab187116)

        Flow cytometry analysis of BOSC23 cells transiently transfected with an expression vector encoding either Claudin 9 (red curve) or a control transfectant; using ab187116 at 1.2 µg/106 cells. PE-conjugated secondary antibodies were used for the analysis.

      参考文献 (0)

      ab187116 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab187116 は論文での使用が確認できていません。

      レビューと Q&A

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      Flow Cytometry abreview for Anti-Claudin 9 antibody [YD4E9]

       Excellent
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (Human CLDN9 overexpression)
      Permeabilization
      No
      Gating Strategy
      Singlets in viable cells
      Specification
      Human CLDN9 overexpression
      Preparation
      Cell harvesting/tissue preparation method: Cells deteched with EDTA without trypsin
      Sample buffer: 2% FBS in PBS
      Fixation
      NO Fixation
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Feb 02 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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