Anti-Claudin 7/CLDN-7 抗体 (ab27487)
Key features and details
- Rabbit polyclonal to Claudin 7/CLDN-7
- Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Claudin 7/CLDN-7 antibody
Claudin 7/CLDN-7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Claudin 7/CLDN-7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Claudin 7/CLDN-7 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95471 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Ab27487 is affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27487の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC | (1) |
Use at an assay dependent concentration.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
|
IHC-P | (3) |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 21525338
|
特記事項 |
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ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. |
ICC/IF
Use at an assay dependent concentration. PubMed: 21525338 |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space. -
組織特異性
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions. -
配列類似性
Belongs to the claudin family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction. - Information by UniProt
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参照データベース
- Entrez Gene: 1366 Human
- Entrez Gene: 53624 Mouse
- Entrez Gene: 65132 Rat
- Omim: 609131 Human
- SwissProt: O95471 Human
- SwissProt: Q9Z261 Mouse
- SwissProt: Q9Z1L1 Rat
- Unigene: 513915 Human
see all -
別名
- CEPTR L2 antibody
- CEPTRL 2 antibody
- CEPTRL2 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab27487 は 40 報の論文で使用されています。
- Xu L et al. Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State. J Oncol 2022:2750193 (2022). PubMed: 35432533
- Wu J et al. Limosilactobacillus reuteri SLZX19-12 Protects the Colon from Infection by Enhancing Stability of the Gut Microbiota and Barrier Integrity and Reducing Inflammation. Microbiol Spectr 10:e0212421 (2022). PubMed: 35658572
- Ueda M et al. Aeromonas sobria Serine Protease Degrades Several Protein Components of Tight Junctions and Assists Bacterial Translocation Across the T84 Monolayer. Front Cell Infect Microbiol 12:824547 (2022). PubMed: 35273923
- Wang K et al. Cldn-7 deficiency promotes experimental colitis and associated carcinogenesis by regulating intestinal epithelial integrity. Oncoimmunology 10:1923910 (2021). PubMed: 34026335
- Schaefer J et al. Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model. Mol Hum Reprod 27:N/A (2021). PubMed: 34524460