AB214576

Anti-Claudin 7/CLDN-7 抗体 [EPR18073] - BSA and Azide free

Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Claudin 7 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

CEPTRL2, CPETRL2, CLDN7, Claudin-7, CLDN-7

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on human renal clear cell carcinoma tissue is observed.

Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

ImmunoFluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on SW480 cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows : --ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on human endometrial cancer tissue is observed.

Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on SW480 cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human renal oncocytoma tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on human renal oncocytoma tissue is observed.

Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

This data was developed using ab207300, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Membranous staining on MCF7 cell line is observed.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18073

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra), IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab214576 is the carrier-free version of ab207300.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 7 often referred to as CLDN-7 is a transmembrane protein with a molecular mass of approximately 22 kDa. It is part of the claudin family which includes other members like claudin-1 and claudin-3. CLDN-7 is prominently found in epithelial cells and plays an important role in forming tight junctions between cells. These tight junctions are integral in maintaining cell polarity and controlling the paracellular transport of ions and molecules.

Biological function summary

Claudin 7 contributes to the integrity and function of tight junctions by directly interacting with other claudins and zonula occludens-1 (ZO-1) thereby becoming part of a larger complex. This structure regulates the movement of substances between epithelial cells protecting tissues from pathogens and maintaining homeostasis. Claudin 7 is essential for proper barrier function in tissues like the respiratory tract and renal structures.

Pathways

The function of claudin 7 integrates within the epithelial barrier signaling pathway and the Wnt signaling pathway. Through these pathways CLDN-7 interacts with proteins like β-catenin. In the epithelial barrier pathway CLDN-7 helps maintain cellular adhesion and barrier function while in the Wnt pathway it might influence cell proliferation and differentiation indirectly through associated proteins.

Claudin 7 has a significant connection with certain cancers such as gastric and colorectal cancer. Alterations in CLDN-7 expression can disrupt cell adhesion leading to increased invasiveness of cancer cells. It is often observed that in these cancers changes in CLDN-7 expression correlate with E-cadherin another key protein in maintaining tissue architecture and integrity. Additionally variations in CLDN-7 are linked to renal disorders where disrupted tight junctions can affect kidney function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a major role in tight junction-specific obliteration of the intercellular space.

See full target information CLDN7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:117 PubMed40670831

2025

ZMIZ1 lactylation induces tamoxifen resistance in breast cancer through increasing transcriptional activity of Nanog to impact cell stemness and cholesterol uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Liu,Jingyu Chen,Li Ma,Shu Zhao,Xue Hui,Wenjing Xiong,Shaoqiang Cheng,Yue Zhang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Claudin 7/CLDN-7 抗体 [EPR18073] - BSA and Azide free

Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Flow Cytometry (Intracellular) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise