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AB265583

Anti-Claudin 7/CLDN-7 抗体

Anti-Claudin 7/CLDN-7 antibody

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(1 Publication)

Rabbit Polyclonal Claudin 7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CLDN7 aa 150 to C-terminus.

別名を表示する

CEPTRL2, CPETRL2, CLDN7, Claudin-7, CLDN-7

2 Images
Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (AB265583)
  • IP

Supplier Data

Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (AB265583)

CLDN7 was immuoprecipitated from 1 mg MCF7 whole cell lysate using ab265583 at 40 μL per reaction. Western blot was performed on the immunoprecipitate using ab265583 at 1/1000 dilution.

Lane 1 : ab265583 IP in MCF7 whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-Claudin 7/CLDN-7 antibody (ab265583)

Predicted band size: 22 kDa

true

Exposure time: 10s

Western blot - Anti-Claudin 7/CLDN-7 antibody (AB265583)
  • WB

Supplier Data

Western blot - Anti-Claudin 7/CLDN-7 antibody (AB265583)

All lanes:

Western blot - Anti-Claudin 7/CLDN-7 antibody (ab265583) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 15 µg

Lane 2:

LNCap whole cell lysate at 15 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg

Predicted band size: 22 kDa

true

Exposure time: 75s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human CLDN7 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95471

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20-40 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 7 often referred to as CLDN-7 is a transmembrane protein with a molecular mass of approximately 22 kDa. It is part of the claudin family which includes other members like claudin-1 and claudin-3. CLDN-7 is prominently found in epithelial cells and plays an important role in forming tight junctions between cells. These tight junctions are integral in maintaining cell polarity and controlling the paracellular transport of ions and molecules.
Biological function summary

Claudin 7 contributes to the integrity and function of tight junctions by directly interacting with other claudins and zonula occludens-1 (ZO-1) thereby becoming part of a larger complex. This structure regulates the movement of substances between epithelial cells protecting tissues from pathogens and maintaining homeostasis. Claudin 7 is essential for proper barrier function in tissues like the respiratory tract and renal structures.

Pathways

The function of claudin 7 integrates within the epithelial barrier signaling pathway and the Wnt signaling pathway. Through these pathways CLDN-7 interacts with proteins like β-catenin. In the epithelial barrier pathway CLDN-7 helps maintain cellular adhesion and barrier function while in the Wnt pathway it might influence cell proliferation and differentiation indirectly through associated proteins.

Claudin 7 has a significant connection with certain cancers such as gastric and colorectal cancer. Alterations in CLDN-7 expression can disrupt cell adhesion leading to increased invasiveness of cancer cells. It is often observed that in these cancers changes in CLDN-7 expression correlate with E-cadherin another key protein in maintaining tissue architecture and integrity. Additionally variations in CLDN-7 are linked to renal disorders where disrupted tight junctions can affect kidney function.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a major role in tight junction-specific obliteration of the intercellular space.
See full target information CLDN7
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:7 PubMed34815759

2021

IRF2-induced Claudin-7 suppresses cell proliferation, invasion and migration of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Weidong Yang
View all publications
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代替製品

Primary Antibodies

AB27487

Anti-Claudin 7/CLDN-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody (AB27487)

  • 4
  • 12
Primary Antibodies

AB207300

Anti-Claudin 7/CLDN-7 antibody [EPR18073]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] (AB207300)

  • 4
  • 2
Primary Antibodies

AB214576

Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (AB214576)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB211737

Anti-Claudin 1 antibody [EPRR18871]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [EPRR18871] (AB211737)

  • 5
  • 7
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB210796

Anti-Claudin 4 antibody [EPRR17575]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (AB210796)

  • 5
  • 2

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