Anti-Claudin 6 抗体 (ab75055)
Key features and details
- Rabbit polyclonal to Claudin 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Claudin 6 antibody
Claudin 6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Claudin 6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from an internal sequence of human Claudin 6.
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ポジティブ･コントロール
- human ovarian tissue, HUVEC cells, extracts from Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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| IHC-P | (2) |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9074 Human
- Omim: 615798 Human
- SwissProt: P56747 Human
- Unigene: 533779 Human
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別名
- Claudin-6 antibody
- Claudin6 antibody
- CLD6_HUMAN antibody
see all
画像
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Western blot - Anti-Claudin 6 antibody (ab75055)Anti-Claudin 6 antibody (ab75055) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 6 antibody (ab75055)Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 6 antibody (ab75055)Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide. -
Western blot - Anti-Claudin 6 antibody (ab75055)All lanes : Anti-Claudin 6 antibody (ab75055) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: <19 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75055 は 2 報の論文で使用されています。
- Florimond A et al. Human hepatic stellate cells are not permissive for hepatitis C virus entry and replication. Gut N/A:N/A (2014). Human . PubMed: 25063678
- Sarhan MA et al. Differential expression of candidate virus receptors in human T lymphocytes prone or resistant to infection with patient-derived hepatitis C virus. PLoS One 8:e62159 (2013). WB ; Human . PubMed: 23626783