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リコンビナントRabMAb

Anti-Claudin 17 抗体 [EPR15483] (ab186744)

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Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
  • Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
  • Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
  • Anti-Claudin 17 antibody [EPR15483] (ab186744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15483] to Claudin 17
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Claudin 17 antibody [EPR15483]
    Claudin 17 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15483] to Claudin 17

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human muscle and fetal kidney lysates; Rat kidney, spleen and Raw 264.7 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15483

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186744の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 関連性

    Claudin proteins are a family of transmembrane proteins associated with tight junctions, and different tissues exhibit different Claudin composition. There are 18 homolgs present in humans, and mutations in any of these proteins cause a variety of human disease, this indicates their physiological importance. CLDN17, clustered with CLDN8 at human chromosome 21q22.11, is a four transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C.

  • 細胞内局在

    Integral membrane protein. Tight junctions.

  • 参照データベース

    • 別名

      • CLDN17 antibody
      • Human CLDN17 gene for claudin 17 antibody

    画像

    • Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Anti-Claudin 17 antibody [EPR15483] (ab186744) at 1/10000 dilution + Human muscle lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Anti-Claudin 17 antibody [EPR15483] (ab186744) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Western blot - Anti-Claudin 17 antibody [EPR15483] (ab186744)
      All lanes : Anti-Claudin 17 antibody [EPR15483] (ab186744) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : Raw 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Anti-Claudin 17 antibody [EPR15483] (ab186744)
      Anti-Claudin 17 antibody [EPR15483] (ab186744)

    参考文献 (0)

    ab186744 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186744 は論文での使用が確認できていません。

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