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Anti-Claudin 14 抗体 (ab19035)

Reviews (2)Q&A (2)References (3)
Western blot - Anti-Claudin 14 antibody (ab19035)

    Key features and details

    • Goat polyclonal to Claudin 14
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Claudin 14 antibody

    • 製品の詳細

      Goat polyclonal to Claudin 14

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Rat, Cow, Dog, Pig

    • 免疫原

      Synthetic peptide:

      SATHSGYRLNDYV

      , corresponding to C terminal amino acids 227-239 of Human Claudin14.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab19035の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).

    1 hour primary incubation is recommended for this product.
    特記事項
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

    • 組織特異性

      Liver, kidney. Also found in ear.

    • 関連疾患

      Defects in CLDN14 are the cause of deafness autosomal recessive type 29 (DFNB29) [MIM:605608]. DFNB29 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

    • 配列類似性

      Belongs to the claudin family.

    • 細胞内局在

      Cell junction > tight junction. Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AI851731 antibody
        • Claudin 14 antibody
        • Claudin-14 antibody
        • CLD14_HUMAN antibody
        • CLDN 14 antibody
        • CLDN14 antibody
        • DFNB 29 antibody
        • DFNB29 antibody
        • Human CLDN14 gene antibody
        • MGC109472 antibody
        • OTTHUMP00000109046 antibody
        • OTTHUMP00000109049 antibody
        • OTTMUSP00000021531 antibody
        • UNQ777/PRO1571 antibody
        see all

      画像

      • Western blot - Anti-Claudin 14 antibody (ab19035)
        Western blot - Anti-Claudin 14 antibody (ab19035)

        ab19035 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence.

        ab19035 (0.3 µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

      参考文献 (3)

      ab19035 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab19035 は 3 報の論文で使用されています。

      • Bolatkan A  et al. Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. Int J Oncol 60:N/A (2022). PubMed: 34913069
      • Jia H  et al. Identification of claudin-2, -6, -11 and -14 as prognostic markers in human breast carcinoma. Int J Clin Exp Pathol 12:2195-2204 (2019). PubMed: 31934042
      • Sato M  et al. Identification of Transcription Factor E3 (TFE3) as a Receptor-independent Activator of G{alpha}16: GENE REGULATION BY NUCLEAR G{alpha} SUBUNIT AND ITS ACTIVATOR. J Biol Chem 286:17766-76 (2011). IHC-P ; Mouse . PubMed: 21454667

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-Claudin 14 antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (15%)
      Sample
      Mouse Tissue lysate - whole (lung)
      Specification
      lung
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Mar 16 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Claudin 14 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (kidney)
      Specification
      kidney
      Fixative
      Methanol
      Permeabilization
      Yes - Triton X
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 5%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 05 2012

      Question

      thank you for your fast answer - this result is also what I suspected. I wanted to ask you how you consider an antibody to fit to an antigen - is there some application or some value. I am aligning the synthetic peptides with ClustalW to my aminoacid sequence but I do not know when something "fits" or not. I would be very thankful if you could give me some short clue or direction. Once again, thank you!

      Read More
      Answer

      Thank you for getting back to me. Generally if an immunogen forms an alignment of 6 or more consecutive residues I would consider it possible that the antibody would react against the homolog of the species. However, if it is a polyclonal antisera this is not always the case as given its polyclonal nature it is a mixture of immunoglobulin molecules. I would be very interested to know the results of your sequence comparisons as I could not find an immunogen that carried significant sequence similarity to warrant the suggestion of the application of the antibody to fish. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      I am searching for a polyclonal antibody which would recognize the following fish specific isoform of claudin with the sequence: MVSAGLQMLGTALAIIGWLGSIIICALPMWKVTAFIGANIVTAQVIWEGLWMNCVTQSTGQMQCKVYDSLLA LPQDLQAARALIIIAIIAGVFAILLGIAGGKCTNFVDNERSKAKVAIASGVVFLIAALLVLVPVCWSANTII RDFYNPLLVEAQRRELGASLYIGWGSAGLMILGGALLCCSCPPKDENHNVKYSKAASSVAGSSKAYV Do you have a claudin antibody which would recognize this protein? Thank you very much in advance!

      Read More
      Answer

      Thank you for your enquiry. I have performed a sequence analysis of your fish sequence with all of immunogens used to raise our antibodies against Claudin. Unfortunately none had significant sequence similarity that would make me consider reactivity likely. I am sorry that I cannot be of more assistance on this occasion.

      Read More

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