Anti-Clathrin light chain 抗体 (ab77542)
Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Clathrin light chain antibody
Clathrin light chain 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Clathrin light chain -
由来種
Goat -
特異性
Expected to recognize all reported isoforms of light chains a and b. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
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免疫原
Synthetic peptide:
CDFNPKSSKQAKD
from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1) -
ポジティブ･コントロール
- Recombinant Human Clathrin light chain protein (ab126693) can be used as a positive control in WB. Human frontal cortex lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77542の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
細胞内局在
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
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参照データベース
- Entrez Gene: 416226 Chicken
- Entrez Gene: 427284 Chicken
- Entrez Gene: 281078 Cow
- Entrez Gene: 281079 Cow
- Entrez Gene: 281698 Cow
- Entrez Gene: 474765 Dog
- Entrez Gene: 489104 Dog
- Entrez Gene: 100055375 Horse
see all -
別名
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
画像
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Western blot - Anti-Clathrin light chain antibody (ab77542)Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 35 kDa (possible isoform)
The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab77542 は 1 報の論文で使用されています。
- Liu H et al. Clathrin-mediated endocytosis in living host cells visualized through quantum dot labeling of infectious hematopoietic necrosis virus. J Virol 85:6252-62 (2011). WB . PubMed: 21525360