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リコンビナントRabMAb

Anti-Clathrin heavy chain 抗体 [EPR12235(B)] (ab172958)

Submit a review Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
  • Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
  • Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Clathrin heavy chain antibody [EPR12235(B)]
    Clathrin heavy chain 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow

  • 免疫原

    Synthetic peptide within Human Clathrin heavy chain aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q00610

  • ポジティブ･コントロール

    • Hela, SH-SY5Y, A431, NIH/3T3, 293T cell lysates, Human colonic carcinoma tissue, Human kidney tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172958の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 191 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 191 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

  • 配列類似性

    Belongs to the clathrin heavy chain family.

  • 細胞内局在

    Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CHC17 antibody
    • Clathrin heavy chain antibody
    • Clathrin heavy chain 1 antibody
    • Clathrin heavy chain 2 antibody
    • Clathrin heavy chain like 1 antibody
    • Clathrin heavy chain on chromosome 17 antibody
    • Clathrin heavy polypeptide antibody
    • CLH 17 antibody
    • CLH 22 antibody
    • CLH-17 antibody
    • CLH1_HUMAN antibody
    • CLH17 antibody
    • CLH22 antibody
    • CLTC antibody
    • CLTCL antibody
    • CLTCL1 antibody
    • CLTCL2 antibody
    • CLTD antibody
    • Hc antibody
    • KIAA0034 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

    Confocal image showing cytoplasmic staining in A431 cells.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.

    Confocal image showing cytoplasmic staining in NIH/3T3 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    All lanes : Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 191 kDa

  • Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
    Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)

参考文献 (6)

ab172958 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab172958 は 6 報の論文で使用されています。

  • Liang T  et al. Enamel defects in Acp4R110C/R110C mice and human ACP4 mutations. Sci Rep 12:16477 (2022). PubMed: 36183038
  • Guo D  et al. Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition. Development 147:N/A (2020). PubMed: 33144400
  • Sui X  et al. METTL3-mediated m6A is required for murine oocyte maturation and maternal-to-zygotic transition. Cell Cycle 19:391-404 (2020). PubMed: 31916488
  • Wang C  et al. Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation. PLoS Pathog 15:e1007938 (2019). PubMed: 31356638
  • Hua Y  et al. Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/ß-catenin pathway. J Biol Chem 293:19710-19724 (2018). PubMed: 30361437
  • Bridgewater RE  et al. Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells. Sci Rep 7:4572 (2017). WB . PubMed: 28676702

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