Anti-Clathrin heavy chain 抗体

• WB

Unknown

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

Blocking buffer : 2% BSA

Gel type : TA

Exposure Time : 1 minute

All lanes:

Western blot - Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

A431 whole cell lysate at 10 µg

Lane 3:

NIH 3T3 whole cell lysate at 10 µg

Lane 4:

PC12 whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 191 kDa

Observed band size: 180 kDa

false

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (AB21679)

ab21679 staining Clathrin heavy chain in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21679 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

AbReview43953****

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (AB21679)

Immunocytochemical analysis of mouse bEnd.3 labeling Clathrin heavy chain with ab21679 at 1/300 dilution using secondary anti-rabbit-alexa fluor 488 at 1/500 dilution.

This image is courtesy of an anonymous Abreview

• ICC/IF

AbReview15420****

Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (AB21679)

ab21679 staining Clathrin heavy chain in mouse embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with -20°C ethanol. Samples were incubated with primary antibody at 1/500 dilution (in 0.1% Saponin/1% BSA/PBS) for 1 hour. An Alexa Fluor^® 546-conjugated goat polyclonal to rabbit IgG (H+L) was used as secondary antibody at 1/500 dilution. Green color in image show positive staining with Alexa Fluor^® 546 conjugated secondary.

This image is a courtesy of Anonymous Abreview

• WB

AbReview43911****

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

All lanes:

Western blot - Anti-Clathrin heavy chain antibody (ab21679) at 1/1000 dilution

All lanes:

HUVEC cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 191 kDa

true

Exposure time: 5s

This image is courtesy of an anonymous Abreview