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Anti-Claspin 抗体 (ab3720)

Reviews (5)Q&A (1)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)

    Key features and details

    • Rabbit polyclonal to Claspin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Claspin antibody
      Claspin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Claspin

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Chimpanzee, Gorilla, Orangutan

    • 免疫原

      Synthetic peptide (Human) - which represented a portion of human Claspin encoded in part by exons 11, 12 and 13.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3720の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

    • 配列類似性

      Belongs to the claspin family.

    • ドメイン

      The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

    • 翻訳後修飾

      Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Claspin antibody
        • claspin homolog (Xenopus laevis) antibody
        • Claspin homolog antibody
        • CLSPN antibody
        • CLSPN protein antibody
        • CLSPN_HUMAN antibody
        • hClaspin antibody
        • Hu Claspin antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.

      参考文献 (16)

      ab3720 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3720 は 16 報の論文で使用されています。

      • Cai C  et al. Claspin Overexpression Promotes Tumor Progression and Predicts Poor Clinical Outcome in Prostate Cancer. Genet Test Mol Biomarkers 25:131-139 (2021). PubMed: 33596143
      • Babasaki T  et al. Overexpression of claspin promotes docetaxel resistance and is associated with prostate-specific antigen recurrence in prostate cancer. Cancer Med 10:5574-5588 (2021). PubMed: 34240817
      • Kobayashi G  et al. Claspin overexpression is associated with high-grade histology and poor prognosis in renal cell carcinoma. Cancer Sci 111:1020-1027 (2020). PubMed: 31912588
      • Rai R  et al. The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1 Independently of MRN Function. Cell Rep 29:3708-3725.e5 (2019). PubMed: 31825846
      • Liang X  et al. Anti-TGF-ß Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria. PLoS One 11:e0155534 (2016). IHC . PubMed: 27187580
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Claspin antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      40 µg
      Gel Running Conditions
      Reduced Denaturing (4-12% Bis-Tris)
      Sample
      Human Cell lysate - nuclear (HeLa nuclear cell lysate)
      Specification
      HeLa nuclear cell lysate
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 08 2013

      Western blot abreview for Anti-Claspin antibody

       Average
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK 293)
      Loading amount
      20 µg
      Specification
      HEK 293
      Gel Running Conditions
      Non-reduced Denaturing (7.5)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Nov 07 2011

      Immunoprecipitation abreview for Anti-Claspin antibody

       Good
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (HT29 tumour cell line)
      Total protein in input
      500 µg
      Specification
      HT29 tumour cell line
      Treatment
      4Gy Ionising Radiation
      Immuno-precipitation step
      Protein A/G
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 13 2009

      Western blot abreview for Anti-Claspin antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (ht29 tumour cell line)
      Loading amount
      50 µg
      Specification
      ht29 tumour cell line
      Treatment
      4Gy Ionising Radiation
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Paul Eve

      Verified customer

      投稿 Aug 07 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Claspin antibody

       Average
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% TritonX100 in PBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Kirk Mcmanus

      Verified customer

      投稿 May 28 2009

      Question

      I recently bought this ab, which, as written in the datasheet, detects a band of approximately 175 kDa. I completed a western blot analysis in Whole human cell lysate (MDA-MB-231 breast cancer cells) treated with increasing doses of the proteasomal inhibitor MG-132 and detected various bands; ˜270, ˜240, ˜175, ˜160, ˜125, ˜100 and ˜70 KDa. Previously, I was using an anti-Claspin antibody (from another company) that was detecting a band at ˜220kDa in these cell line. Since my strongest bands are the 270 and 240 kDa, I was wondering whether this is the actual claspin band(s) and not the 175/160 kDa?

      Also, has anybody else reported detecting two bands? It seems that I detect a lot of double bands.

      ·

      Read More
      Answer

      The predicted MW of the protein is 151 kDa for isoform 1. Two other isoforms exist, isoform 2 (144 kDa) and isoform 3 (150 kDa) (see http://www.uniprot.org/uniprot/Q9HAW4), but I am not sure under which conditions you would be able to detect either one and in which samples they might be present. The literature might be able to help in this regard.

      As for your WB results, the band should be around 175 - 180 kDa that's where the lab and other customers detected the band (see Abreviews). I cannot find an explanation for the 2 bands around 250 kDa as well as the other bands you see around 130, 100 and 70 kDa. I believe these must be non-specific bands.

      Read More

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