Anti-CKAP4 抗体 (ab152154)
Key features and details
- Rabbit polyclonal to CKAP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CKAP4 antibody
CKAP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CKAP4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 245-573 of CKAP4 (UniProt ID: Q07065).
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ポジティブ･コントロール
- A549, HeLa, HepG2, HCT116 and MDA-MB-231 whole cell lysate; HeLa cells; CL1-5 xenograft tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152154の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 66 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 66 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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翻訳後修飾
Reversibly palmitoylated. -
細胞内局在
Endoplasmic reticulum-Golgi intermediate compartment membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10970 Human
- SwissProt: Q07065 Human
- Unigene: 74368 Human
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別名
- 63 kDa membrane protein antibody
- CKAP 4 antibody
- Ckap4 antibody
see all
画像
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Western blot - Anti-CKAP4 antibody (ab152154)Anti-CKAP4 antibody (ab152154) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 66 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CKAP4 antibody (ab152154)Immunofluorescence analysis of methanol fixed HeLa cells, labeling CKAP4 using ab152154 at 1/200 dilution. Lower image costained with Hoerchst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP4 antibody (ab152154)Immunohistochemical analysis of paraffin embedded CL1-5 xenograft tissue, labeling CKAP4 using ab152154 at 1/500 dilution. -
Western blot - Anti-CKAP4 antibody (ab152154)Anti-CKAP4 antibody (ab152154) at 1/2000 dilution + MDA-MB-231 whole cell lysate at 30 µg with 5% non-fat milk in TBST
Predicted band size: 66 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab152154 は 2 報の論文で使用されています。
- Vaklavas C et al. Hallmarks and Determinants of Oncogenic Translation Revealed by Ribosome Profiling in Models of Breast Cancer. Transl Oncol 13:452-470 (2020). PubMed: 31911279
- Vaklavas C et al. Translational control of the undifferentiated phenotype in ER-positive breast tumor cells: Cytoplasmic localization of ERa and impact of IRES inhibition. Oncol Rep 39:2482-2498 (2018). PubMed: 29620220