Anti-CKAP1/TBCB 抗体 (ab96101)
Key features and details
- Rabbit polyclonal to CKAP1/TBCB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CKAP1/TBCB antibody
CKAP1/TBCB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CKAP1/TBCB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human CKAP1/TBCB aa 1-213.
Database link: NP_001272 -
ポジティブ･コントロール
- WB: A431, H1299, HeLa and NIH/3T3 whle cell lysates. IHC-P: H1299 xenograft ICC/IF: A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96101の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth. -
組織特異性
Found in most tissues. -
配列類似性
Belongs to the TBCB family.
Contains 1 CAP-Gly domain. -
翻訳後修飾
Phosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone. - Information by UniProt
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参照データベース
- Entrez Gene: 1155 Human
- Entrez Gene: 66411 Mouse
- Entrez Gene: 292777 Rat
- Omim: 601303 Human
- SwissProt: Q99426 Human
- SwissProt: Q9D1E6 Mouse
- Unigene: 31053 Human
- Unigene: 27947 Mouse
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別名
- CG22 antibody
- CKAPI antibody
- Cytoskeleton associated protein 1 antibody
see all
画像
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Western blot - Anti-CKAP1/TBCB antibody (ab96101)All lanes : Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CKAP1/TBCB antibody (ab96101)Methanol-fixed A549 (human lung carcinoma cell line) cells labeling CKAP1/TBCB using ab96101 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKAP1/TBCB antibody (ab96101)ab96101, at a 1/500 dilution, staining CKAP1/TBCB in paraffin embedded H1299 xenograft by Immunohistochemical analysis.
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Western blot - Anti-CKAP1/TBCB antibody (ab96101)Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96101 は 1 報の論文で使用されています。
- Gong J et al. Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomed Rep 6:525-531 (2017). PubMed: 28515911