Anti-CK1 epsilon 抗体 (ab115293)
Key features and details
- Goat polyclonal to CK1 epsilon
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CK1 epsilon antibody
CK1 epsilon 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CK1 epsilon -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Chicken, Pig, Xenopus laevis, Monkey, Gorilla, Common marmoset, Xenopus tropicalis, Bat
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免疫原
Synthetic peptide corresponding to Human CK1 epsilon aa 400-500 (C terminal) (Cysteine residue).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 98% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115293の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 47 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 47 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation. -
組織特異性
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes. -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 378891 Chicken
- Entrez Gene: 1454 Human
- Omim: 600863 Human
- SwissProt: Q5ZLL1 Chicken
- SwissProt: P49674 Human
- Unigene: 474833 Human
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別名
- Casein kinase 1 epsilon antibody
- Casein kinase I antibody
- Casein kinase I epsilon antibody
see all
画像
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Western blot - Anti-CK1 epsilon antibody (ab115293)Anti-CK1 epsilon antibody (ab115293) at 1 µg/ml + HEK293 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 1 hour -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CK1 epsilon antibody (ab115293)Human tonsil (formalin-fixed, paraffin-embedded) stained with ab115293 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab115293 は 6 報の論文で使用されています。
- Huang Q et al. Role of CK1ε-regulated PERIOD2 in STZ-induced diabetic myocardial injury. Front Biosci (Landmark Ed) 27:58 (2022). PubMed: 35227001
- Jiang T et al. Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide. Tissue Eng Regen Med 19:403-415 (2022). PubMed: 35122584
- Xue X et al. YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription. Cell Death Discov 8:59 (2022). PubMed: 35149670
- Wang H et al. Forskolin exerts anticancer roles in non-Hodgkin's lymphomas via regulating Axin/ß-catenin signaling pathway. Cancer Manag Res 11:1685-1696 (2019). PubMed: 30863177
- Wang D et al. MiR-182 promotes prostate cancer progression through activating Wnt/ß-catenin signal pathway. Biomed Pharmacother 99:334-339 (2018). WB . PubMed: 29353209
- Yang WH et al. Heterogeneous nuclear ribonucleoprotein M promotes the progression of breast cancer by regulating the axin/ß-catenin signaling pathway. Biomed Pharmacother 105:848-855 (2018). PubMed: 30021377