Anti-CIP2A 抗体 (ab84547)
Key features and details
- Rabbit polyclonal to CIP2A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CIP2A antibody
CIP2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CIP2A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CIP2A aa 550-650. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8TCG1 -
ポジティブ･コントロール
- IHC-P: Human colon carcinoma. Human ovarian carcinoma.
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特記事項
Concentration is optimized for IHC and not determined.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. -
組織特異性
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). -
細胞内局在
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 57650 Human
- Omim: 610643 Human
- SwissProt: Q8TCG1 Human
- Unigene: 591308 Human
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別名
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIP2A antibody (ab84547)
Detection of Human CIP2A by Immunohistochemistry.Sample: FFPE section of Human colon carcinoma.Antibody: ab84547 used at a dilution of 1/250.Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIP2A antibody (ab84547)Detection of Human CIP2A by Immunohistochemistry.Sample: FFPE section of Human ovarian carcinoma.Antibody: ab84547 used at a dilution of 1/250.Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIP2A antibody (ab84547)Image from Tseng LM et al., Breast Cancer Res. 2012 Apr 26;14(2):R68 Fig 6.; doi:10.1186/bcr3175; 26 April 2012, Breast Cancer Research 2012, 14:R68Immunohistochemical analysis of Human breast tumour tissue, staining CIP2A with ab84547 at 1/100 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab84547 は 10 報の論文で使用されています。
- Xu Z et al. Depletion of CIP2A inhibits the proliferation, migration, invasion and epithelial-mesenchymal transition of glioma cells. Brain Res Bull 173:14-21 (2021). PubMed: 33892085
- Xu J et al. Overexpression of S100A4 Predicts Migration, Invasion, and Poor Prognosis of Hypopharyngeal Squamous Cell Carcinoma. Mol Diagn Ther N/A:N/A (2019). PubMed: 30868407
- Liu CY et al. Targeting SET to restore PP2A activity disrupts an oncogenic CIP2A-feedforward loop and impairs triple negative breast cancer progression. EBioMedicine 40:263-275 (2019). PubMed: 30651219
- Huang YH et al. SET Overexpression is Associated with Worse Recurrence-Free Survival in Patients with Primary Breast Cancer Receiving Adjuvant Tamoxifen Treatment. J Clin Med 7:N/A (2018). PubMed: 30154367
- Cha G et al. High expression of CIP2A protein is associated with tumor aggressiveness in stage I-III NSCLC and correlates with poor prognosis. Onco Targets Ther 10:5907-5914 (2017). PubMed: 29276394
- Liu CY et al. Carfilzomib induces leukaemia cell apoptosis via inhibiting ELK1/KIAA1524 (Elk-1/CIP2A) and activating PP2A not related to proteasome inhibition. Br J Haematol 177:726-740 (2017). PubMed: 28340282
- Liu CY et al. Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A dependent phospho-Akt inactivation in estrogen-receptor negative human breast cancer cells. Breast Cancer Res 16:431 (2014). IHC-P . PubMed: 25228280
- Yu HC et al. Erlotinib derivative inhibits hepatocellular carcinoma by targeting CIP2A to reactivate protein phosphatase 2A. Cell Death Dis 5:e1359 (2014). IHC-P . PubMed: 25077545
- Tseng LM et al. CIP2A is a target of bortezomib in human triple negative breast cancer cells. Breast Cancer Res 14:R68 (2012). IHC-P ; Human . PubMed: 22537901
- Ren J et al. Expression of CIP2A in renal cell carcinomas correlates with tumour invasion, metastasis and patients' survival. Br J Cancer 105:1905-11 (2011). ICC/IF ; Human . PubMed: 22075943