Anti-Cingulin 抗体 (ab117796)
Key features and details
- Rabbit polyclonal to Cingulin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Cingulin antibody
Cingulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cingulin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide, corresponding to a region between amino acids 200-250 of Human Cingulin (NP_065821.1).
-
ポジティブ･コントロール
- 293T, Jurkat, and HeLa whole cell lysate (ab150035)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117796の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 136 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
-
機能
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. -
組織特異性
Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart. -
配列類似性
Belongs to the cingulin family. -
ドメイン
Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1. -
細胞内局在
Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions. - Information by UniProt
-
参照データベース
- Entrez Gene: 57530 Human
- Omim: 609473 Human
- SwissProt: Q9P2M7 Human
- Unigene: 591464 Human
-
別名
- 6330408J11Rik antibody
- AI647528 antibody
- AI987749 antibody
see all
画像
-
Western blot - Anti-Cingulin antibody (ab117796)All lanes : Anti-Cingulin antibody (ab117796) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg/ml
Lane 2 : 293T whole cell lysate at 15 µg/ml
Lane 3 : Jurkat whole cell lysate at 50 µg/ml
Lane 4 : HeLa whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 136 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-Cingulin antibody (ab117796)ab117796 at 1 µg/ml staining Cingulin in 293T cell lysate immunoprecipitated using ab117796 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab117796 は 1 報の論文で使用されています。
- Soroosh A et al. miR-24 Is Elevated in Ulcerative Colitis Patients and Regulates Intestinal Epithelial Barrier Function. Am J Pathol 189:1763-1774 (2019). PubMed: 31220450