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リコンビナントRabMAb

Anti-ChT1 抗体 [EPR9356] (ab154186)

Reviews (2) Submit a question References (1)
Western blot - Anti-ChT1 antibody [EPR9356] (ab154186)
  • Anti-ChT1 antibody [EPR9356] (ab154186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9356] to ChT1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ChT1 antibody [EPR9356]
    ChT1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9356] to ChT1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human stomach, Human oesophagus cancer cell, Human ovary cancer and BxPC-3 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9356

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 組織特異性

    Detected only in stomach mucosa and testis, and to a much lesser level in pancreas (at protein level). Detected in gastric cancers (31%), esophageal carcinomas (50%) and ovarian cancers (23%).

  • 配列類似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    Highly N-glycosylated. Appears not to contain significant amounts of O-linked carbohydrates or sialic acid in its sugar moieties.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 1700062D20Rik antibody
    • Cell surface A33 antigen antibody
    • ChT1 thymocyte antigen antibody
    • dJ889N15.1 antibody
    • Glycoprotein A34 antibody
    • GPA34 antibody
    • MGC44287 antibody
    • OTTHUMP00000023820 antibody
    • V set and immunoglobulin domain containing antibody
    • V-set and immunoglobulin domain containing 1 antibody
    • V-set and immunoglobulin domain-containing protein 1 antibody
    • VSIG1 antibody
    • VSIG1_HUMAN antibody
    see all

画像

  • Western blot - Anti-ChT1 antibody [EPR9356] (ab154186)
    Western blot - Anti-ChT1 antibody [EPR9356] (ab154186)
    All lanes : Anti-ChT1 antibody [EPR9356] (ab154186) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human esophagus cancer lysate
    Lane 3 : Human ovary cancer lysate
    Lane 4 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 42 kDa

  • Anti-ChT1 antibody [EPR9356] (ab154186)
    Anti-ChT1 antibody [EPR9356] (ab154186)

参考文献 (1)

ab154186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154186 は 1 報の論文で使用されています。

  • Yoon EJ  et al. Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF. Int J Mol Sci 23:N/A (2022). PubMed: 35628371

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-ChT1 antibody [EPR9356]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Dog Tissue sections (Atria)
Specification
Atria
Permeabilization
Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Juan Estrada

Verified customer

投稿 Jul 29 2014

Western blot abreview for Anti-ChT1 [EPR9356] antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
8 µg
Gel Running Conditions
Reduced Denaturing (4% stacking, 7.5% resolving)
Sample
Dog Tissue lysate - whole (atria)
Specification
atria
Blocking step
KPL Signa Lock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 02 2014

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