Anti-Chromogranin C/SGII 抗体 [SGII 6B1/3] (ab20245)
![Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)](https://www.abcam.co.jp/ps/products/20/ab20245/Images/ab20245-29542-anti-chromogranin-c-sgii-antibody-sgii-6b1-3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)")
Key features and details
- Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: IgG2b
製品の概要
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製品名
Anti-Chromogranin C/SGII antibody [SGII 6B1/3]
Chromogranin C/SGII 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII -
由来種
Mouse -
特異性
SGII6B1/3 has potential uses for chromatogranin diagnostics. -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to Rat Chromogranin C/SGII (C terminal).
Database link: P10362 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
SGII 6B1/3 -
ミエローマ
Sp2 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20245の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 24765 Rat
- SwissProt: P10362 Rat
- Unigene: 45602 Rat
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別名
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)](https://www.abcam.co.jp/ps/products/20/ab20245/Images/ab20245-29541-anti-chromogranin-c-sgii-antibody-sgii-6b1-3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab20245 は 7 報の論文で使用されています。
- Çakmakli GY et al. Striatal Neurotransmitter Release-related Presynaptic Proteins in L-dopa Induced Dyskinesia in a Model of Parkinsonism. Noro Psikiyatr Ars 55:73-79 (2018). PubMed: 30042645
- Chen Y et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). PubMed: 26942217
- Berard AR et al. Differential Reovirus-Specific and Herpesvirus-Specific Activator Protein 1 Activation of Secretogranin II Leads to Altered Virus Secretion. J Virol 89:11954-64 (2015). PubMed: 26378181
- Oh JY et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med : (2012). ICC/IF ; Rat . PubMed: 22359280
- Kim CH et al. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem 286:9196-204 (2011). WB . PubMed: 21209083
- Urbé S et al. Homotypic fusion of immature secretory granules during maturation in a cell-free assay. J Cell Biol 143:1831-44 (1998). PubMed: 9864358
- Dittié AS & Tooze SA Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310 ( Pt 3):777-87 (1995). PubMed: 7575410