Anti-Chromogranin A 抗体 [EP1030Y] (ab68271)
![Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)](https://www.abcam.co.jp/ps/products/68/ab68271/Images/ab68271-82467-anti-chromogranin-a-antibody-ep1030y-western-blot.jpg "Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1030Y] to Chromogranin A
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Chromogranin A antibody [EP1030Y]
Chromogranin A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1030Y] to Chromogranin A -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: SH-SY5Y cell lysate. IHC-P: Human pancreas tissue. ICC/IF: SH-SY5Y. Flow Cyt (intra): SH-SY5Y cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1030Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-Chromogranin A antibody [EP1030Y] (ab199192)
- HRP Anti-Chromogranin A antibody [EP1030Y] (ab199194)
- PE Anti-Chromogranin A antibody [EP1030Y] (ab213341)
- Anti-Chromogranin A antibody [EP1030Y] - BSA and Azide free (ab215276)
- Alexa Fluor® 568 Anti-Chromogranin A antibody [EP1030Y] (ab312978)
- Alexa Fluor® 750 Anti-Chromogranin A antibody [EP1030Y] (ab321690)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 51 kDa).
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| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/250. |
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WB
1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 51 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
翻訳後修飾
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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製品の状態
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)Anti-Chromogranin A antibody [EP1030Y] (ab68271) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Secondary
HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EP1030Y] (ab68271)](https://www.abcam.co.jp/ps/products/68/ab68271/Images/ab68271-3-anti-chromogranin-a-antibody-ep1030y-immunocytochemistry-shsy5y-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EP1030Y] (ab68271)Immunocytochemistry analysis of SH-SY5Y (human neuroblastoma epithelial cell) labeling Chromogranin A with purified ab68271 at 1/50 dilution (3 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.17 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)](https://www.abcam.co.jp/ps/products/68/ab68271/Images/ab68271-82466-anti-chromogranin-a-antibody-ep1030y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)Formalin/PFA-fixed paraffin-embedded human pancreas tissue stained for Chromogranin A with ab68271 at a 1/100 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)](https://www.abcam.co.jp/ps/products/68/ab68271/Images/ab68271-5-anti-chromogranin-a-antibody-ep1030y-flowcytometry-jukat-shsy5y-human.jpg)
Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / SH-SY5Y (Human neuroblastoma epithelial cell, Right) cells labeling Chromogranin A with purified ab68271 at 1/200 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left).
Negative control: Jurkat. -
![Anti-Chromogranin A antibody [EP1030Y] (ab68271)](https://www.abcam.co.jp/ps/products/68/ab68271/Images/ab68271-4-benefits-of-recombinant-antibodies.png)
Anti-Chromogranin A antibody [EP1030Y] (ab68271)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab68271 は 14 報の論文で使用されています。
- Almobarak B et al. Exposure to nonanoic acid alters small intestinal neuroendocrine tumor phenotype. BMC Cancer 23:267 (2023). PubMed: 36959559
- Rassol N et al. Co-administration with A1M does not influence apoptotic response of 177Lu-octreotate in GOT1 neuroendocrine tumors. Sci Rep 13:6417 (2023). PubMed: 37076494
- Ji S et al. miR-145-5p Inhibits Neuroendocrine Differentiation and Tumor Growth by Regulating the SOX11/MYCN Axis in Prostate cancer. Front Genet 13:790621 (2022). PubMed: 35368699
- Elvborn M et al. Hyperfractionated Treatment with 177Lu-Octreotate Increases Tumor Response in Human Small-Intestine Neuroendocrine GOT1 Tumor Model. Cancers (Basel) 14:N/A (2022). PubMed: 35008397
- Shapiro AMJ et al. Insulin expression and C-peptide in type 1 diabetes subjects implanted with stem cell-derived pancreatic endoderm cells in an encapsulation device. Cell Rep Med 2:100466 (2021). PubMed: 35028608